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Sol-Gel Optical Sensors for Glutamate

Published online by Cambridge University Press:  15 March 2011

Jenna L. Rickus
Affiliation:
NeuroengineeringUniversity of California, Los Angeles Los Angeles, CA 90095, U.S.A
Esther Lan
Affiliation:
Department of Materials Science and EngineeringUniversity of California, Los Angeles Los Angeles, CA 90095, U.S.A
Allan J. Tobin
Affiliation:
Brain Research InstituteUniversity of California, Los Angeles Los Angeles, CA 90095, U.S.A
Jeffery I. Zink
Affiliation:
Department of Chemistry and BiochemistryUniversity of California, Los Angeles Los Angeles, CA 90095, U.S.A
Bruce Dunn
Affiliation:
Department of Materials Science and EngineeringUniversity of California, Los Angeles Los Angeles, CA 90095, U.S.A
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Abstract

The amino acid glutamate is the major excitatory neurotransmitter used in the nervous system for interneuronal communication. It is used throughout the brain by various neuronal pathways including those involved in learning and memory, locomotion, and sensory perception. Because glutamate is released from neurons on a millisecond time scale into sub-micrometer spaces, the development of a glutamate biosensor with high temporal and spatial resolution is of great interest for the study of neurological function and disease. Here, we demonstrate the feasibility of an optical glutamate sensor based on the sol-gel encapsulation of the enzyme glutamate dehydrogenase (GDH). GDH catalyses the oxidative deamination of glutamate and the reduction of NAD+ to NADH. NADH fluorescence is the basis of the sensor detection. Thermodynamic and kinetic studies show that GDH remains active in the sol-gel matrix and that the reaction rate is correlated to the glutamate concentration.

Type
Research Article
Copyright
Copyright © Materials Research Society 2001

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References

1. Kandel, E.R., in Essentials of Neural Science and Behavior, edited by Kandel, E.R., Schwartz, J.H., Jessell, T.M. (Appleton & Lange, Connecticut, 1995), pp. 2139.Google Scholar
2. Westerink, B.H.C., Behav. Brain Res. 70, 103124 (1995).Google Scholar
3. O'Neill, R.D., Lowry, J.P., Mas, M., Critical Rev. Neurobio. pp.12, 69–127 (1998).Google Scholar
4. Hösing, N., Reisler, E., Zink, J.I., J. Sol-Gel Sci. Tech. 15, pp. 5761 (1999).Google Scholar
5. Sharma, A. and Schulman, S.G., Introduction to Fluorescence Spectroscopy, 1st ed. (John Wiley and Sons, Inc. New York, 1999) p.145.Google Scholar
6. Lehninger, A.L., Nelson, D.L., Cox, M.M., Principles of Biochemistry, 2nd ed. (Worth Publishers, New York, 1993), pp. 212218.Google Scholar
7. Subramanian, S., Biophys. Chem. 7, 375378 (1978).Google Scholar