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Tissue Engineered Growth of New Cartilage in the Shape of a Human Ear Using Synthetic Polymers Seeded with Chondrocytes

Published online by Cambridge University Press:  15 February 2011

Charles A. Vacanti ,
Linda G. Cima ,
Dianne Ratkowski ,
Joseph Upton  and
Joseph P. Vacanti
Charles A. Vacanti
Affiliation:
Mass. General Hospital, Department of Anaesthesia, Boston, MA 02114
Linda G. Cima
Affiliation:
Massachusetts Institute of Technology, Chemical Engineering Dept., Cambridge, MA 02139
Dianne Ratkowski
Affiliation:
Boston University, Department of Otolaryngology, Boston, MA 02114
Joseph Upton
Affiliation:
Children's Hospital, Department of Surgery, Boston, MA, 02115.
Joseph P. Vacanti
Affiliation:
Children's Hospital, Department of Surgery, Boston, MA, 02115.
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Abstract

This report concerns the tissue-engineered growth of new cartilage in the shape of a human ear. Using synthetic biodegradable polyesters, a porous, three dimensional device in the shape of a human ear was fabricated. The polymer matrices were seeded with living chondrocytes isolated from a freshly sacrificed calf shoulder and implanted subcutaneously on the dorsum of athymic rats. This resulted in the formation of new cartilage in the shape of a human ear of approximately the same dimensions as the original implants. Histological analysis revealed the presence of mature cartilage in all specimens.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 252: Symposium T – Tissue-Inducing Biomaterials , 1991 , 367
Copyright
Copyright © Materials Research Society 1992

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References

Literature Cited

1. Cima, L.G., Ingber, D., Vacanti, C., and Vacanti, J., 1991, “Polymer matrices for cell transplantation and organ regeneration” J.Biomech Eng., 113, 143–151.Google Scholar
2. Vacanti, C. A, Langer, R., Schloo, B., Vacanti, J.P., 1991, “Synthetic biodegradable polymers seeded with chondrocytes provide a template for new cartilage formation,” J.Plastic Reconstr. Surgery, 88(5)Google ScholarPubMed
3. Singhal, J.P., Singh, H., and Ray, A.R., 1988, “Absorbable suture materials: preparation and properties,” Rev. Macromol. Chem. Phys, C28, 475–502.Google Scholar
4. Gilding, D.K., and Reed, A.M., 1979, “Biodegradable polymers for use in surgery– polyglycolic/poly(lactic acid) homo-and copolymers” Polymer, 20, 1459–1464.Google Scholar
5. Craig, P.H., Williams, J.A., Davis, K.W., Magoun, A.D., Levy, A.J., Bogdansky, S., and Jones, J.P. Jr., 1975, “A biologic comparison of polyglactin 910 and polyglycolic acid synthetic absorbable sutures,” Surg., Gyn. Obs., 141, 1–10.Google Scholar
6. Klagsburn, M., 1979, “Large-scale preparation of chondrocytes,” Meth. Enzym., 58, 560–564.Google Scholar
7. Chu, C.C., 1982, “The effect of pH on the in vitro degradation of poly(glycolide lactide) copolymer absorbable sutures,” J. Biomed. Mat. Res., 16, 117–124.Google Scholar
8. Williams, D.F., 1982, “Review: Biodegradation of surgical polymers,” J. Mat. Sci., 17, 1233–1246.Google Scholar
9. Greisler, H.P., Dennis, J.W., Endean, E.D., Ellinger, J., Buttle, K.F., and Kim, D.U. 1988, “Derivation of neointima in vascular grafts,” Am.J Phys., Supp I, Circulation, 78(3) 16–112. Google Scholar
10. Gogolewski, S., and Pennings, A.J. 1983 “Resorbable materials of poly(L-lactide) III. Porous materials for medical application,” Colloid and Polymer Sci., 261, 477–484.Google Scholar