Genetical Research



Microsatellite isolation, linkage group identification and determination of recombination frequency in the peach-potato aphid, Myzus persicae (Sulzer) (Hemiptera: Aphididae)


MATHEW A.  SLOANE  a1, PAUL  SUNNUCKS  a2, ALEX C. C.  WILSON  a3 and DINAH F.  HALES  a1 c1
a1 Department of Biological Sciences, Macquarie University, NSW 2109, Australia
a2 Department of Genetics, La Trobe University, VIC 3083, Australia
a3 Division of Botany and Zoology, The Australian National University, ACT 0200, Australia

Abstract

Fifteen polymorphic microsatellite markers were used to establish linkage groups and relative rates of recombination in male and female Myzus persicae (Sulzer) (Hemiptera: Aphididae) (peach-potato aphid). We cloned nine markers from M. persicae and for these we report primer sequences and levels of allelic diversity and heterozygosity in four Australian M. persicae populations. Of the remaining six loci, four loci, also cloned from M. persicae, were obtained from G. Malarky (Natural History Museum, London) and two loci from Sitobion miscanthi were used. Additionally, the primer sequences of locus M77, a locus monomorphic in M. persicae but polymorphic in the closely related Myzus antirrhinii, are presented. Eleven of the 15 polymorphic markers were autosomal and four were X-linked. A linkage analysis was performed on a European pedigree of aphids containing five families with between seven and 11 offspring each. There was no linkage between any loci in females. In males, several pairwise comparisons yielded no recombinant offspring. With the exception of locus M40, these observations were supported in a linkage analysis performed on larger families produced from Australian M. persicae crosses. Locus M40 showed segregation consistent with involvement in a translocation between autosomes 1 and 3 in European samples but not in the Australian samples. From the Australian crosses we report an absence of recombination in males but high recombination rates in females. One X chromosome and four autosomal linkage groups were identified and tentatively assigned to chromosomes. The relevance of achiasmate meiosis to the evolution of sex is discussed.

(Received August 31 2001)
(Revised January 5 2001)


Correspondence:
c1 Corresponding author. Telephone: (612) 9850 8181. Fax: (612) 9850 8245. e-mail: dhales@rna.bio.mq.edu.au.


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