Genetical Research



PGal4 excision reveals the pleiotropic effects of Voila, a Drosophila locus that affects development and courtship behaviour


YAEL  GROSJEAN  a1, MARIA  BALAKIREVA  a2, LAURENCE  DARTEVELLE  a1 and JEAN-FRANCOIS  FERVEUR  a1 c1
a1 Développement et Communication Chimique, CNRS-UMR 5548, Faculté des Sciences, Université de Bourgogne, 6, Bd Gabriel, 21000 Dijon, France
a2 Centre des Sciences du Goût, 21000 Dijon, France

Abstract

In Drosophila melanogaster, the PGal4 transposon inserted at the chromosomal site 86E1-2 is associated with the Voila1 allele that causes multiple phenotypes. Homozygous Voila1/1 flies rarely reach adulthood and heterozygous Voila1/+ adult males display strong homosexual courtship behaviour. Both normal behavioural and developmental phenotypes were rescued by remobilizing the PGal4 element. Yet, the rescue of heterosexual courtship and of adult viability did not occur in the same strains, indicating that these defects have different genetic origins. Furthermore, many strains showed a partial rescue of both characters. Molecular analysis revealed that the PGal4 transposon is inserted upstream of the 5′UTR of the prospero gene. The excision strains with no detectable fragment of the PGal4 transposon remaining showed a rescued viability for homozygote adults. Moreover, the developmental period with the highest homozygote lethality was correlated with the size of PGal4 element that remained inserted at the Voila locus. This suggests a relationship between developmental viability and the amount of DNA inserted within the promoter of prospero.

(Received August 28 2000)
(Revised November 27 2000)


Correspondence:
c1 Corresponding author. Tel. +33 (0) 3 80 39 37 82 (office), +33 (0) 3 80 39 62 19 (lab). Fax: +33 (0) 3 80 39 62 89. e-mail: jean-francois.ferveur@u-bourgogne.fr


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