MRS Proceedings

Table of Contents - Volume  1093  - Symposium CC – Designer Biointerfaces  

Editors : E. Chaikof, A. Chilkoti, J. Elisseeff, J. Lahann

Articles

Production of Highly Ordered Nanoporous Alumina and its Application in Cell Cultivation

2008 MRS Spring Meeting.

Andreas Hoessa1, Andrea Staeudtea2, Annika Thormanna3, Martin Steinharta4 and Andreas Heilmanna5

a1 andreas.hoess@iwmh.fraunhofer.de, Fraunhofer Institute for Mechanics of Materials, Biological Materials and Interfaces, Walter-Hülse-Strasse 1, Halle, 06120, Germany, +49345 5589286, +49345 5589101

a2 andrea.staeudte@iwmh.fraunhofer.de, Fraunhofer Institute for Mechanics of Materials, Biological Materials and Interfaces, Walter-Hülse-Strasse 1, Halle, 06120, Germany

a3 annika.thormann@iwmh.fraunhofer.de, Fraunhofer Institute for Mechanics of Materials, Biological Materials and Interfaces, Walter-Hülse-Strasse 1, Halle, 06120, Germany

a4 steinhar@mpi-halle.mpg.de, Max Planck Institute of Microstructure Physics, Experimental Department II, Weinberg 2, Halle, 06120, Germany

a5 andreas.heilmann@iwmh.fraunhofer.de, Fraunhofer Institute for Mechanics of Materials, Biological Materials and Interfaces, Walter-Hülse-Strasse 1, Halle, 06120, Germany

Abstract

Nanoporous alumina membranes were utilized as cell culture substrates. Two different anodization processes were applied in order to obtain highly ordered, self-supporting nanoporous alumina membranes. The resulting membranes exhibit uniform nanopores with constant diameters having aspect ratios (pore depth:pore diameter) larger than 1000. Tentative experiments evaluating the cellular interaction of a hepatoma cell line (HepG2) with the nanoporous alumina membranes will be described. Furthermore, cell proliferation as well as the adhesion behavior of the cells on the porous substrates was investigated. Scanning electron microscopy (SEM) and focused ion beam (FIB) technology were applied to examine the cell morphology. The experiments revealed that the nanoporous membranes can be used as cell culture substrates and that they have no adverse effects on cell proliferation and cell viability. Besides, cells on membranes with pore diameters larger than 200 nm developed small cell extensions (filopodia) which penetrated into the nanopores.

(Received September 12 2008)

(Accepted October 09 2008)

Key Words

  • nanostructure;
  • biomaterial;
  • porosity
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