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First insights into the biochemistry of Sabella spallanzanii (Annelida: Polychaeta) mucus: a potentially unexplored resource for applicative purposes

Published online by Cambridge University Press:  14 July 2010

Loredana Stabili ,
Roberto Schirosi ,
Angela Di Benedetto ,
Alessandro Merendino ,
Luciano Villanova  and
Adriana Giangrande
Loredana Stabili*
Affiliation:
Istituto per l'Ambiente Marino Costiero, Sezione di Taranto Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Università del Saliento, 73100 (Lecce), Italy
Roberto Schirosi
Affiliation:
Istituto per l'Ambiente Marino Costiero, Sezione di Taranto
Angela Di Benedetto
Affiliation:
Dipartimento di Chimica e CIRCC, Università degli Studi di Bari, Campus Universitario, 70126 Bari, Italy
Alessandro Merendino
Affiliation:
Lachifarma s.r.l. Laboratorio Chimico Farmaceutico, Dipartimento Ricerca & Sviluppo S.S. 16 Zona Industriale, 73010 Zollino (Lecce), Italy
Luciano Villanova
Affiliation:
Lachifarma s.r.l. Laboratorio Chimico Farmaceutico, Dipartimento Ricerca & Sviluppo S.S. 16 Zona Industriale, 73010 Zollino (Lecce), Italy
Adriana Giangrande
Affiliation:
Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Università del Saliento, 73100 (Lecce), Italy
*
Correspondence should be addressed to: L. Stabili, Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Università del Salento, 73100 (Lecce), Italy email: loredana.stabili@iamc.cnr.it
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Abstract

Although mucus plays many different roles among marine invertebrates, relatively little is known about the link between biochemical structure and function. In the present study we focused on some physical and chemical properties of the polychaete Sabella spallanzanii's mucus such as viscosity, osmolarity, electrical conductivity, elemental composition, the protein and carbohydrate content, the total lipids and fatty acid composition, and polychlorinated biphenyls (PCBs) contamination. Moreover, an antimicrobial activity of the mucus was investigated. The water content of S. spallanzanii mucus was 96.2±0.3%. By dry weight 26±1.2% was protein, 8±0.21% was carbohydrate and only 0.1% lipid, much of the remainder of the dry weight was inorganic (about 65.2%). The estimated PCBs content was <0.005 μg g−1. The mucus of S. spallanzanii exerted a natural lysozyme-like activity and produced in vitro the growth inhibition of Vibrio anguillarum, Vibrio harveyi, Pseudomonas aeruginosa and Candida albicans. The findings from this study contribute to improve the limited knowledge available on the mucus composition in invertebrates and have implications for future investigations related to employment of S. spallanzanii mucus as a source of compounds of pharmaceutical and marine technological interest.

Keywords

mucusproteinscarbohydrateslipidsPCBslysozymeantimicrobial activity
Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 91 , Issue 1 , February 2011 , pp. 199 - 208
Copyright
Copyright © Marine Biological Association of the United Kingdom 2010

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