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Bioinspired synthesis of self-assembled calcium phosphate nanocomposites using block copolymer-peptide conjugates

Published online by Cambridge University Press:  31 January 2011

Yusuf Yusufoglu ,
Yanyan Hu ,
Mathumai Kanapathipillai ,
Matthew Kramer ,
Yunus E. Kalay ,
P. Thiyagarajan ,
Mufit Akinc ,
Klaus Schmidt-Rohr  and
Surya Mallapragada
Yusuf Yusufoglu
Affiliation:
Ames Laboratory, Ames, Iowa 50011
Yanyan Hu
Affiliation:
Ames Laboratory, Ames, Iowa 50011
Mathumai Kanapathipillai
Affiliation:
Ames Laboratory, Ames, Iowa 50011
Matthew Kramer
Affiliation:
Ames Laboratory, Ames, Iowa 50011
Yunus E. Kalay
Affiliation:
Ames Laboratory, Ames, Iowa 50011
P. Thiyagarajan
Affiliation:
Advanced Photon Source, Argonne National Laboratory, Argonne, Illinois 60439
Mufit Akinc
Affiliation:
Ames Laboratory, Ames, Iowa 50011
Klaus Schmidt-Rohr
Affiliation:
Ames Laboratory, Ames, Iowa 50011
Surya Mallapragada*
Affiliation:
Ames Laboratory, Ames, Iowa 50011
*
b)Address all correspondence to this author. suryakm@iastate.edu
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Abstract

Thermoreversibly gelling block copolymers conjugated to hydroxyapatite-nucleating peptides were used to template the growth of inorganic calcium phosphate in aqueous solutions. Nuclear magnetic resonance (NMR), Fourier transform infrared (FTIR), transmission electron microscopy, x-ray diffraction, and small-angle scattering were used to characterize these samples and confirm that the peptides promoted the growth of hydroxyapatite as the inorganic phase. Three different polymer templates were used with varying charges on the polymer chains (nonionic, anionic, and zwitterionic), to investigate the role of charge on mineralization. All of the polymer-inorganic solutions exhibited thermoreversible gelation above room temperature. Nanocomposite formation was confirmed by solid-state NMR, and several methods identified the inorganic component as hydroxyapatite. Small angle x-ray scattering and electron microscopy showed thin, elongated crystallites. Thermogravimetric analysis showed an inorganic content of 30–45 wt% (based on the mass of the dried gel at ∼200 °C) in the various samples. Our work offers routes for bioinspired bottom-up approaches for the development of novel, self-assembling, injectable nanocomposite biomaterials for potential orthopedic applications.

Keywords

Biomimetic (assembly)NanostructureSelf-assembly
Type
Articles
Information
Journal of Materials Research , Volume 23 , Issue 12: Biomimetic and Bio-enabled Materials Science and Engineering , December 2008 , pp. 3196 - 3212
Copyright
Copyright © Materials Research Society 2008

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