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Population dynamics of the liver fluke, Fasciola hepatica: the effect of time and spatial separation on the genetic diversity of fluke populations in the Netherlands

Published online by Cambridge University Press:  27 August 2010

S. M. WALKER ,
C. JOHNSTON ,
E. M. HOEY ,
I. FAIRWEATHER ,
F. BORGSTEEDE ,
C. GAASENBEEK ,
P. A. PRODÖHL  and
A. TRUDGETT
S. M. WALKER
Affiliation:
School of Biological Sciences, The Queen's University of Belfast, 97 Lisburn Road, Belfast N. Ireland
C. JOHNSTON
Affiliation:
School of Biological Sciences, The Queen's University of Belfast, 97 Lisburn Road, Belfast N. Ireland
E. M. HOEY
Affiliation:
School of Biological Sciences, The Queen's University of Belfast, 97 Lisburn Road, Belfast N. Ireland
I. FAIRWEATHER
Affiliation:
School of Biological Sciences, The Queen's University of Belfast, 97 Lisburn Road, Belfast N. Ireland
F. BORGSTEEDE
Affiliation:
Animal Sciences Group, Wageningen UR, Edelhertweg 15, 8219 PH Lelystad, The Netherlands
C. GAASENBEEK
Affiliation:
Animal Sciences Group, Wageningen UR, Edelhertweg 15, 8219 PH Lelystad, The Netherlands
P. A. PRODÖHL
Affiliation:
School of Biological Sciences, The Queen's University of Belfast, 97 Lisburn Road, Belfast N. Ireland
A. TRUDGETT*
Affiliation:
School of Biological Sciences, The Queen's University of Belfast, 97 Lisburn Road, Belfast N. Ireland
*
*Corresponding author: School of Biological Sciences, The Queen's University of Belfast, 97 Lisburn Road, Belfast N. Ireland. Tel: +44 028 9097 2125. Fax: +44 028 9097 5877. E-mail: a.trudgett@qub.ac.uk
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Summary

An evaluation of the genetic diversity within Fasciola hepatica (liver fluke) may provide an insight into its potential to respond to environmental changes, such as anthelmintic use or climate change. In this study, we determined the mitochondrial DNA haplotypes of >400 flukes from 29 individual cattle, from 2 farms in the Netherlands, as an exemplar of fasciolosis in a European context. Analysis of this dataset has provided us with a measure of the genetic variation within infrapopulations (individual hosts) and the diversity between infrapopulations within a herd of cattle. Temporal sampling from one farm allowed for the measurement of the stability of genetic variation at a single location, whilst the comparison between the two farms provided information on the variation in relation to distance and previous anthelmintic regimes. We showed that the liver fluke population in this region is predominantly linked to 2 distinct clades. Individual infrapopulations contain a leptokurtic distribution of genetically diverse flukes. The haplotypes present on a farm have been shown to change significantly over a relatively short time-period.

Keywords

Fasciola hepaticagenetic diversitymitochondrial haplotypesbiogeographytrematode
Type
Research Article
Information
Parasitology , Volume 138 , Issue 2 , February 2011 , pp. 215 - 223
Copyright
Copyright © Cambridge University Press 2010

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