a1 firstname.lastname@example.org, Johns Hopkins University, Baltimore, Maryland, United States
a2 email@example.com, Johns Hopkins University, Baltimore, Maryland, United States
a3 firstname.lastname@example.org, Johns Hopkins University, Baltimore, Maryland, United States
a4 email@example.com, Johns Hopkins University, Chemical and Biomolecular Engineering, 3400 N Charles Street, 125 Maryland Hall, Baltimore, Maryland, 21218, United States, 410-516-5284, 410-516-5510
In many biological applications, such as cell therapy and drug delivery, there is a need to enhance diffusion by enabling chemical transport in all three dimensions. We highlight this need by comparing diffusion in a conventional two-dimensional (2D) microwell with diffusion in a three-dimensional (3D) cubic microwell using numerical simulations. We also describe the fabrication of hollow polymeric (and biocompatible) cubic microwells and microwell arrays. We emphasize that since the assembly process is compatible with 2D lithographic patterning, porosity can be precisely patterned in all three dimensions. Hence, this platform provides considerable versatility for a variety of applications.
(Received April 21 2010)
(Accepted September 15 2010)