British Journal of Nutrition

Table of Contents - 2011 - Volume 105, Issue 01  

Full Papers

Molecular Nutrition

Relationship between proteome changes of Longissimus muscle and intramuscular fat content in finishing pigs fed conjugated linoleic acid

Weijing Zhonga1a2, Zongyong Jianga1 c1, Chuntian Zhenga1 c1, Yingcai Lina1, Lin Yanga3 and Shutong Zoua3

a1 Ministry of Agriculture Key Laboratory of Animal Nutrition and Feed Science (South China), State Key Laboratory of Livestock and Poultry Breeding, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, People's Republic of China

a2 College of Life Science, South China Normal University, Guangzhou 510631, People's Republic of China

a3 College of Animal Science, South China Agricultural University, Guangzhou 510642, People's Republic of China

Abstract

The present experiment was conducted to determine proteome changes in Longissimus muscle of finishing pigs fed conjugated linoleic acid (CLA), in association with alteration of intramuscular fat content. Previously, seventy-two Duroc × Landrace × Large White gilts (approximately 60 kg) had been fed maize–soyabean meal-based diets with 0, 12·5 and 25 g CLA/kg diet. The CLA contained 369·1 mg/g cis-9, trans-11 CLA, 374·6 mg/g trans-10, cis-12 CLA and 53·7 mg/g other isomers. Six pigs per treatment were slaughtered when they reached a body weight of approximately 100 kg. Data published from a previous experiment demonstrated that supplementation with 12·5 or 25 g CLA/kg diet increased intramuscular fat content (P < 0·05). The present study investigated the proteome changes in Longissimus muscle of control and pigs supplemented with 25 g CLA/kg diet. CLA significantly influenced the abundance of proteins related to energy metabolism, fatty acid oxidation and synthesis, amino acid metabolism, defence, transport and other miscellaneous processes (P < 0·05). The increase in intramuscular fat content was positively correlated with the increased abundance of carbonic anhydrase 3 and aspartate aminotransferase (P < 0·05). We suggest that the proteome changes in Longissimus muscle contributed to greater intramuscular lipid content in CLA-supplemented pigs.

(Received November 03 2009)

(Revised July 16 2010)

(Accepted July 22 2010)

(Online publication September 01 2010)

Key Words:

Correspondence:

c1 Corresponding authors: Dr Z. Jiang, email jiangz38@gmail.com; C. Zheng, email zhengcht@gmail.com

Footnotes

Abbreviations: AMPK-γ3, 5′-AMP-activated protein kinase subunit gamma-3; AST, aspartate aminotransferase; CA3, carbonic anhydrase 3; CLA, conjugated linoleic acid; LCFA, long-chain fatty acids; MCAD, medium-chain specific acyl-CoA dehydrogenase; SCD, stearoyl-CoA desaturase