Parasitology

Research Article

Influence of host reproductive state on Sphaerothecum destruens prevalence and infection level

D. ANDREOUa1 c1, M. HUSSEYa2, S. W. GRIFFITHSa1 and R. E. GOZLANa3

a1 Cardiff School of Biosciences, Cardiff University, Biomedical Sciences Building, Museum Avenue, Cardiff CF10 3AX, UK

a2 Centre for Ecology and Hydrology, Mansfield Road, Oxford OX1 3SR, UK

a3 School of Conservation Sciences, Bournemouth University, Poole BH12 5BB, Dorset, UK

SUMMARY

Sphaerothecum destruens is an obligate intracellular parasite with the potential to cause high mortalities and spawning inhibition in the endangered cyprinid Leucaspius delineatus. We investigated the influence of L. delineatus’s reproductive state on the prevalence and infection level of S. destruens. A novel real time quantitative polymerarse chain reaction (qPCR) was developed to determine S. destruens’ prevalence and infection level. These parameters were quantified and compared in reproductive and non-reproductive L. delineatus. The detection limit of the S. destruens specific qPCR was determined to be 1 pg of purified S. destruens genomic DNA. Following cohabitation in the lab, reproductive L. delineatus had a significantly higher S. destruens prevalence (P<0·05) and infection levels (P<0·01) compared to non-reproductive L. delineatus. S. destruens prevalence was 19% (n=40) in non-reproductive L. delineatus and 41% (n=32) in reproductive L. delineatus. However, there was no difference in S. destruens prevalence in reproductive and non-reproductive fish under field conditions. Mean infection levels were 18 and 99 pg S. destruens DNA per 250 ng L. delineatus DNA for non-reproductive and reproductive L. delineatus respectively. The present work indicates that S. destruens infection in L. delineatus can be influenced by the latter's reproductive state and provides further support for the potential adverse impact of S. destruens on the conservation of L. delineatus populations.

(Received March 11 2010)

(Revised May 17 2010)

(Accepted June 03 2010)

(Online publication July 21 2010)

Correspondence:

c1 Corresponding author: School of Conservation Sciences, Bournemouth University, Poole BH12 5BB, UK. Tel: +44 1202 965 268. Fax: +44 1202 965 046. E-mail: dandreou@bournemouth.ac.uk

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