British Journal of Nutrition

Full Papers

Human and Clinical Nutrition

Effects of dietary fat modification on oxidative stress and inflammatory markers in the LIPGENE study

Helena Peterssona1, Ulf Risérusa1 c1, Jolene McMonaglea2, Hanne L. Gulsetha3a4, Audrey C. Tierneya2, Sophie Morangea5, Olfa Helala6a7a8, Danielle I. Shawa9, Juan A. Ruanoa10, José López-Mirandaa10, Beata Kieć-Wilka11, Iwona Gołąbeka11, Ellen E. Blaaka12, Wim H. M. Sarisa12, Christian A. Drevona4, Julie A. Lovegrovea9a13, Helen M. Rochea2 and Samar Basua1

a1 Department of Public Health and Caring Sciences/Clinical Nutrition and Metabolism, Uppsala University, Uppsala Science Park, 75185 Uppsala, Sweden

a2 Nutrigenomics Research Group, UCD Conway Institute, School of Public Health and Population Science, University College Dublin, Dublin 4, Ireland

a3 Hormone Laboratory, Department of Endocrinology, Oslo University Hospital Aker, University of Oslo, Oslo, Norway

a4 Department of Nutrition, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, Oslo, Norway

a5 Centre d'Investigation Clinique, Aix-Marseille Université, APH-M, Hôpital Conception, Marseille, France

a6 INSERM, U476 Nutrition Humaine et Lipides, Marseille F-13385, France

a7 INRA, UMR1260 Nutriments Lipidiques et Prévention des Maladies Métaboliques, Marseille F-13385, France

a8 Université de la Méditerranée Aix-Marseille 2, Faculté de Médecine, Marseille F-13385, France

a9 Department of Food and Nutritional Sciences, University of Reading, Reading, UK

a10 Lipids and Atherosclerosis Research Unit, Reina Sofía University Hospital, Maimonides Institute for Biomedical Research at Cordoba (IMIBIC), University of Cordoba, Ciber Physiopatology of Obesity and Nutrition (CB06/03), Instituto de Salud Carlos III, Cordoba, Spain

a11 Department of Clinical Biochemistry, Jagiellonian University Medical College, Krakow, Poland

a12 Department of Human Biology, NUTRIM, School for Metabolism, Toxicology and Nutrition, Maastricht University, Maastricht, The Netherlands

a13 Institute for Cardiovascular and Metabolic Research (ICMR), University of Reading, Reading, UK

Abstract

Subjects with the metabolic syndrome (MetS) have enhanced oxidative stress and inflammation. Dietary fat quality has been proposed to be implicated in these conditions. We investigated the impact of four diets distinct in fat quantity and quality on 8-iso-PGF (a major F2-isoprostane and oxidative stress indicator), 15-keto-13,14-dihydro-PGF (15-keto-dihydro-PGF, a major PGF metabolite and marker of cyclooxygenase-mediated inflammation) and C-reactive protein (CRP). In a 12-week parallel multicentre dietary intervention study (LIPGENE), 417 volunteers with the MetS were randomly assigned to one of the four diets: two high-fat diets (38 % energy (%E)) rich in SFA or MUFA and two low-fat high-complex carbohydrate diets (28 %E) with (LFHCC n-3) or without (LFHCC) 1·24 g/d of very long chain n-3 fatty acid supplementation. Urinary levels of 8-iso-PGF and 15-keto-dihydro-PGF were determined by RIA and adjusted for urinary creatinine levels. Serum concentration of CRP was measured by ELISA. Neither concentrations of 8-iso-PGF and 15-keto-dihydro-PGF nor those of CRP differed between diet groups at baseline (P>0·07) or at the end of the study (P>0·44). Also, no differences in changes of the markers were observed between the diet groups (8-iso-PGF, P = 0·83; 15-keto-dihydro-PGF, P = 0·45; and CRP, P = 0·97). In conclusion, a 12-week dietary fat modification did not affect the investigated markers of oxidative stress and inflammation among subjects with the MetS in the LIPGENE study.

(Received December 09 2009)

(Revised April 13 2010)

(Accepted April 21 2010)

(Online publication June 23 2010)

Correspondence:

c1 Corresponding author: Dr U. Risérus, fax +46 18 611 79 76, email ulf.riserus@pubcare.uu.se

Footnotes

Abbreviations: 15-keto-dihydro-PGF, 15-keto-13,14-dihydro-PGF; %E, % energy; CRP, C-reactive protein; DPA, docosapentaenoic acid; HMUFA, high-fat diet rich in MUFA; HSFA, high-fat diet rich in SFA; LFHCC, low-fat high-complex carbohydrate diets; MetS, metabolic syndrome

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