Parasitology

Research Article

A battery of 12 microsatellite markers for genetic analysis of the Leishmania (Viannia) guyanensis complex

V. ROUGERONa1 c1, T. DE MEEÛSa2a3, M. HIDEa1, E. WALECKXa4, J. DEREUREa1a5, J. AREVALOa6, A. LLANOS-CUENTASa6 and A. L. BAÑULSa1

a1 Génétique et Evolution des Maladies Infectieuses, IRD/CNRS/UMI (UMR 2724), Montpellier, F-34394, France

a2 UMR 177 IRD-CIRAD “Laboratoire de Recherches et de Coordination sur les Trypanosomoses”, Centre International de Recherche-Développement sur l'Elevage en zone Subhumide (Cirdes), 01 BP 454, Bobo-Dioulasso 01, Burkina-Faso

a3 CNRS, Délégation Languedoc-Roussillon, 1919, route de Mende – 34293 Montpellier cedex 5, France

a4 UR016 IRD, INLASA, Laboratoire d'entomologie médicale de La Paz, Bolivie

a5 Laboratoire de Parasitologie – Mycologie, Montpellier, F 34090, France

a6 Instituto de medicina Tropical “Alexander von Humboldt,” Lima, Peru

SUMMARY

We used 12 microsatellite markers developed for Leishmania braziliensis to genotype 28 strains of the main species of the Leishmania guyanensis complex (i.e. L. guyanensis and L. panamensis) collected in Ecuador and Peru. The important heterozygote deficits observed in these populations are similar with the previous data obtained in L. braziliensis and raise again the debate on the reproductive mode of these protozoan parasites. The data showed genetic polymorphism and geographical differentiation giving information on population structure of the L. guyanensis complex. Regarding the two species, this study enhances again the debate on the taxonomic status of the different isolates belonging to L. guyanensis s.l. since the results showed substantial heterogeneity within this species complex. In conclusion, this study increases the number of available microsatellite loci for L. guyanensis species complex and raises fundamental biological questions. It confirms that microsatellite markers constitute good tools for population genetic studies on parasites of this complex.

(Received October 6 2009)

(Revised December 27 2009)

(Revised April 07 2010)

(Accepted April 12 2010)

(Online publication July 07 2010)

Correspondence:

c1 Corresponding author: Laboratoire GEMI, UMR IRD/CNRS/UMI 2724, Centre IRD, 911, avenue Agropolis BP 64501, 34394 Montpellier Cedex 5, France. Tel: +33 4 67 41 62 26. Fax: +33 4 67 41 62 99. E-mail: rougeron.virginie@ gmail.com

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