British Journal of Nutrition

Full Papers

Metabolism and Metabolic Studies

Antioxidant responses to an acute ultra-endurance exercise: impact on DNA stability and indications for an increased need for nutritive antioxidants in the early recovery phase

Oliver Neubauera1 c1, Stefanie Reichholda1, Lukas Nicsa1, Christine Hoelzla2, Judit Valentinia1, Barbara Stadlmayra1, Siegfried Knasmüllera2 and Karl-Heinz Wagnera1

a1 Department of Nutritional Sciences, Emerging Field Oxidative Stress and DNA Stability, Faculty of Life Sciences, University of Vienna, Althanstraße 14, 1090 Vienna, Austria

a2 Environmental Toxicology Group, Institute of Cancer Research, Medical University of Vienna, Borschkegasse 8A, 1090 Vienna, Austria

Abstract

Antioxidant requirements have neither been defined for endurance nor been defined for ultra-endurance athletes. To verify whether an acute bout of ultra-endurance exercise modifies the need for nutritive antioxidants, we aimed (1) to investigate the changes of endogenous and exogenous antioxidants in response to an Ironman triathlon; (2) to particularise the relevance of antioxidant responses to the indices of oxidatively damaged blood lipids, blood cell compounds and lymphocyte DNA and (3) to examine whether potential time-points of increased susceptibility to oxidative damage are associated with alterations in the antioxidant status. Blood that was collected from forty-two well-trained male athletes 2 d pre-race, immediately post-race, and 1, 5 and 19 d later was sampled. The key findings of the present study are as follows: (1) Immediately post-race, vitamin C, α-tocopherol, and levels of the Trolox equivalent antioxidant capacity, the ferric reducing ability of plasma and the oxygen radical absorbance capacity (ORAC) assays increased significantly. Exercise-induced changes in the plasma antioxidant capacity were associated with changes in uric acid, bilirubin and vitamin C. (2) Significant inverse correlations between ORAC levels and indices of oxidatively damaged DNA immediately and 1 d post-race suggest a protective role of the acute antioxidant responses in DNA stability. (3) Significant decreases in carotenoids and γ-tocopherol 1 d post-race indicate that the antioxidant intake during the first 24 h of recovery following an acute ultra-endurance exercise requires specific attention. Furthermore, the present study illustrates the importance of a diversified and well-balanced diet to maintain a physiological antioxidant status in ultra-endurance athletes in reference to recommendations.

(Received December 09 2009)

(Revised April 09 2010)

(Accepted April 12 2010)

(Online publication July 19 2010)

Correspondence:

c1 Corresponding author: Dr O. Neubauer, fax +43 1 4277 9549, email oliver.neubauer@univie.ac.at

Footnotes

Abbreviations: AOPP, advanced oxidation protein products; FRAP, ferric reducing ability of plasma; ORAC, oxygen radical absorbance capacity; RONS, reactive oxygen and nitrogen species; TEAC, Trolox equivalent antioxidant capacity

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