British Journal of Nutrition

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British Journal of Nutrition (2010), 104:100-107 Cambridge University Press
Copyright © The Authors 2010

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Human and Clinical Nutrition

The effects of a 30-month dietary intervention on bone mineral density: The Postmenopausal Health Study

George Moschonisa1, Ioanna Katsarolia1, George P. Lyritisa2 and Yannis Maniosa1 c1

a1 Department of Nutrition & Dietetics, Harokopio University of Athens, Athens, Greece
a2 Laboratory for the Research of the Musculoskeletal System, School of Medicine, University of Athens, Athens, Greece
Article author query
moschonis g [PubMed]  [Google Scholar]
katsaroli i [PubMed]  [Google Scholar]
lyritis gp [PubMed]  [Google Scholar]
manios y [PubMed]  [Google Scholar]


Low dietary Ca intake and vitamin D insufficiency have been implicated as part of the aetiology leading to osteoporosis. The aim of the present study was to examine the effects of a 30-month dietary intervention that combined supplementation of dairy products fortified with Ca and vitamin D3 and lifestyle and nutrition counselling sessions on bone mineral density (BMD) of postmenopausal women. Sixty-six postmenopausal women (aged 55–65 years) were randomised into a dietary group (DG; n 35), receiving daily and for the first 12 months 1200 mg Ca and 7·5 μg vitamin D3, while for the next 18 months of intervention 1200 mg Ca and 22·5 μg vitamin D3 through fortified dairy products, and a control group (CG; n 31) receiving neither counselling nor dairy products. The DG was found to have more favourable changes in arms (P < 0·001), total spine (P = 0·001) and total body BMD (P < 0·001) compared with the CG. Furthermore, a significant increase was observed for the DG in lumbar spine BMD (0·056; 95 % CI 0·009, 0·103), which was not found to differentiate significantly compared with the change observed in the CG (P = 0·075). In conclusion, the present study showed that intakes of vitamin D of about 22·5 μg/d and of Ca close to the recommended level of 1200 mg from fortified dairy foods for 30 months, with compliance ensured by lifestyle and nutrition counselling sessions, can induce favourable changes in arms, total spine and total body BMD of postmenopausal women.

(Received June 17 2009)

(Revised January 11 2010)

(Accepted January 12 2010)

(Online publication April 07 2010)

Key Words:Bone mass; Calcium and vitamin D; Fortified milk


c1 Corresponding author: Dr Yannis Manios, fax +30 210 9514759, email


Abbreviations: BMD, bone mineral density; CG, control group; DG, dietary group; DXA, dual-energy X-ray absorptiometry