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Ultrastructural Imaging of Candida albicans Adhesion to Rat Genital Epithelium through Scanning and Transmission Electron Microscopy

Published online by Cambridge University Press:  07 April 2010

Edilson Damke ,
Agenor Storti-Filho ,
Mary M.T. Irie ,
Márcia A. Carrara ,
Marcia R. Batista ,
Lucélia Donatti ,
Luciene S.A. Gunther ,
Eliana V. Patussi ,
Terezinha I.E. Svidzinski  and
Márcia E.L. Consolaro
Edilson Damke
Affiliation:
Department of Clinical Analysis, State University of Maringá, Av. Colombo, 5790, 87020-900, Maringá, Paraná, Brazil
Agenor Storti-Filho
Affiliation:
Department of Clinical Analysis, State University of Maringá, Av. Colombo, 5790, 87020-900, Maringá, Paraná, Brazil
Mary M.T. Irie
Affiliation:
Department of Clinical Analysis, State University of Maringá, Av. Colombo, 5790, 87020-900, Maringá, Paraná, Brazil
Márcia A. Carrara
Affiliation:
Department of Clinical Analysis, State University of Maringá, Av. Colombo, 5790, 87020-900, Maringá, Paraná, Brazil
Marcia R. Batista
Affiliation:
Department of Clinical Analysis, State University of Maringá, Av. Colombo, 5790, 87020-900, Maringá, Paraná, Brazil
Lucélia Donatti
Affiliation:
Department of Cell Biology, Federal University of Paraná, Curitiba, 85131-990, Paraná, Brazil
Luciene S.A. Gunther
Affiliation:
Department of Clinical Analysis, State University of Maringá, Av. Colombo, 5790, 87020-900, Maringá, Paraná, Brazil
Eliana V. Patussi
Affiliation:
Department of Clinical Analysis, State University of Maringá, Av. Colombo, 5790, 87020-900, Maringá, Paraná, Brazil
Terezinha I.E. Svidzinski
Affiliation:
Department of Clinical Analysis, State University of Maringá, Av. Colombo, 5790, 87020-900, Maringá, Paraná, Brazil
Márcia E.L. Consolaro*
Affiliation:
Department of Clinical Analysis, State University of Maringá, Av. Colombo, 5790, 87020-900, Maringá, Paraná, Brazil
*
Corresponding author. E-mail: melconsolaro@yahoo.com.br
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Abstract

The adhesion of Candida albicans to the genital epithelium has not been fully investigated in vivo. The objective of this study was to evaluate the ultrastructural aspects of C. albicans adhesion in the lower genital system of female Wistar rats through scanning and transmission electron microscopy. The genital infection persisted until the end of the experiment, and all rats showed the same adhesion aspects. Various associated yeast/hyphae were observed in the lumen and adhered both at the vaginal and endocervical levels where the fungal filamentation process occurred. In the vaginal epithelium, closely adhered yeasts were observed as stretched strands bridging between yeasts and the epithelium surface. Different stages of the adhesion, where yeasts internalized into the epithelial cell inside a cytoplasmic vacuole, resembling endocytosis, and a wide fibrillar-floccular, glycocalyx-like layer on the yeasts were observed. On the endocervix, the adhesion occurred between the cilia. In the uterine body, only a yeast-like form was observed with superficial contact. This study reached the initial goal of demonstrating an experimental model for in vivo studies. Continuation of this line of research is important for studies of vulvovaginal candidiasis.

Keywords

Candida albicansadhesion in vivoultrastructuregenital epithelium
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 16 , Issue 3 , June 2010 , pp. 337 - 345
Copyright
Copyright © Microscopy Society of America 2010

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