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Effect of postnatal age and a β3-adrenergic agonist (Zeneca D7114) administration on uncoupling protein-1 abundance in the lamb

Published online by Cambridge University Press:  31 July 2001

J. A. Bird ,
A. Mostyn ,
L. Clarke ,
D. T. Juniper ,
H. Budge ,
T. Stephenson  and
M. E. Symonds
J. A. Bird
Affiliation:
Academic Division of Child Health, School of Human Development, University Hospital, Queen's Medical Centre, Nottingham NG7 2UH, The Nightingale Institute, Division of Nursing and Midwifery, School of Life, Basic and Medical Health Sciences, Kings College, London SE1 9RT, Department of Agriculture and Horticulture, Wye College, University of London, Wye, Ashford TN25 5AH and Department of Agriculture, University of Reading, Reading RG 6 6AJ, UK
A. Mostyn
Affiliation:
Academic Division of Child Health, School of Human Development, University Hospital, Queen's Medical Centre, Nottingham NG7 2UH, The Nightingale Institute, Division of Nursing and Midwifery, School of Life, Basic and Medical Health Sciences, Kings College, London SE1 9RT, Department of Agriculture and Horticulture, Wye College, University of London, Wye, Ashford TN25 5AH and Department of Agriculture, University of Reading, Reading RG 6 6AJ, UK
L. Clarke
Affiliation:
Academic Division of Child Health, School of Human Development, University Hospital, Queen's Medical Centre, Nottingham NG7 2UH, The Nightingale Institute, Division of Nursing and Midwifery, School of Life, Basic and Medical Health Sciences, Kings College, London SE1 9RT, Department of Agriculture and Horticulture, Wye College, University of London, Wye, Ashford TN25 5AH and Department of Agriculture, University of Reading, Reading RG 6 6AJ, UK
D. T. Juniper
Affiliation:
Academic Division of Child Health, School of Human Development, University Hospital, Queen's Medical Centre, Nottingham NG7 2UH, The Nightingale Institute, Division of Nursing and Midwifery, School of Life, Basic and Medical Health Sciences, Kings College, London SE1 9RT, Department of Agriculture and Horticulture, Wye College, University of London, Wye, Ashford TN25 5AH and Department of Agriculture, University of Reading, Reading RG 6 6AJ, UK
H. Budge
Affiliation:
Academic Division of Child Health, School of Human Development, University Hospital, Queen's Medical Centre, Nottingham NG7 2UH, The Nightingale Institute, Division of Nursing and Midwifery, School of Life, Basic and Medical Health Sciences, Kings College, London SE1 9RT, Department of Agriculture and Horticulture, Wye College, University of London, Wye, Ashford TN25 5AH and Department of Agriculture, University of Reading, Reading RG 6 6AJ, UK
T. Stephenson
Affiliation:
Academic Division of Child Health, School of Human Development, University Hospital, Queen's Medical Centre, Nottingham NG7 2UH, The Nightingale Institute, Division of Nursing and Midwifery, School of Life, Basic and Medical Health Sciences, Kings College, London SE1 9RT, Department of Agriculture and Horticulture, Wye College, University of London, Wye, Ashford TN25 5AH and Department of Agriculture, University of Reading, Reading RG 6 6AJ, UK
M. E. Symonds
Affiliation:
Academic Division of Child Health, School of Human Development, University Hospital, Queen's Medical Centre, Nottingham NG7 2UH, The Nightingale Institute, Division of Nursing and Midwifery, School of Life, Basic and Medical Health Sciences, Kings College, London SE1 9RT, Department of Agriculture and Horticulture, Wye College, University of London, Wye, Ashford TN25 5AH and Department of Agriculture, University of Reading, Reading RG 6 6AJ, UK
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Abstract

We examined the effect of time after birth and β3-adrenergic agonist (Zeneca D7114) administration on uncoupling protein-1 (UCP1) abundance and thermoregulation in the lamb. Forty twin lambs, all born normally at term, were maintained at a cold ambient temperature of between 3 and 8 °C. At 0.5, 1.75, 5.25, 11.25 and 23.25 h after birth eight sets of twins were fed 20 ml of formula milk ± 10 mg kg-1 of β3-adrenergic agonist, and 45 min after feeding brown adipose tissue (BAT) was sampled. Colonic temperature was measured and BAT analysed for UCP1 abundance, GDP-binding to mitochondrial protein (i.e. thermogenic activity) and catecholamine content. Colonic temperature declined between 1.25 and 6 h from 40.2 °C to 39.2 °C and then increased to 39.8 °C at 12 h, but increased after feeding at all ages. UCP1 abundance increased from 1.25 h after birth, to peak at 2 h after birth in controls, compared with 6 h after birth in β3-adrenergic agonist-treated lambs. The level of GDP-binding to mitochondrial protein did not change significantly with age but was increased by β3-adrenergic agonist treatment. The noradrenaline (norepinephrine) content of BAT increased between 1.25 and 12 h after birth, irrespective of β3-adrenergic agonist administration. The total weight of perirenal BAT plus its lipid, protein and mitochondrial protein content declined over the first 6 h of life. UCP1 development continues over the first 24 h of neonatal life, and can be manipulated by β3-adrenergic agonist administration. This may represent one method of improving thermoregulation in newborn lambs. Experimental Physiology (2001) 86.1, 65-70.

Type
Research Article
Information
Experimental Physiology , Volume 86 , Issue 1 , January 2001 , pp. 65 - 70
Copyright
© The Physiological Society 2001

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