Genetical Research



Research Paper

Dynamics of the hobo transposable element in transgenic lines of Drosophila melanogaster


V.  LADEVEZE  a1, S.  AULARD  a2, N.  CHAMINADE  a2, C.  BIEMONT  a3, G.  PERIQUET  a4 and F.  LEMEUNIER  a2 c1
a1 Laboratoire de Génétique Cellulaire et Moléculaire, Université de Poitiers, CHU, 86021 Poitiers Cedex, France
a2 Laboratoire Populations, Génétique et Evolution, Centre National de la Recherche Scientifique, Avenue de la Terrasse, 91198 Gif-sur-Yvette Cedex, France
a3 Laboratoire de Biométrie et Biologie Evolutive, UMR5558, Université Lyon 1, 69622 Villeurbanne, France
a4 Institut de Recherche sur la Biologie de l'Insecte, Université François Rabelais, Parc Grandmont, 37200 Tours, France

Abstract

The impact of the hobo transposable element in global reorganization of the Drosophila melanogaster genome has been investigated in transgenic lines generated by injection of hobo elements into the Hikone strain, which lacked them. In the present extensive survey, the chromosomal distribution of hobo insertion sites in the line 28 was found to be homogeneous and similar for all chromosomal arms, except 3L, when compared with other transgenic lines. However, some original features were observed in this line at the genetic and chromosomal levels. Several hotspots of insertion sites were observed on the X, second and third chromosomes. Five sites with a high frequency of hobo insertions were present on the 3L arm in most individuals tested, suggesting the action of selection for hobo element in some sites. The presence of doublets or triplet was also observed, implying that hobo inserts can show local jumps or insertions in preferred regions. This local transposition occurred independently in 11 specific genomic regions in many individuals and generations. The dynamics of this phenomenon were analysed across generations. These results support the use of the hobo system as an important tool in fundamental and applied Drosophila genetics.

(Received July 10 2000)
(Revised November 20 2000)


Correspondence:
c1 Corresponding author. Tel : +33 1 69 82 37 07. Fax : +33 1 69 07 04 21. e-mail : Francoise.Lemeunier@pge.cnrs-gif.fr.


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