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A Simple Method for Storing Mosquito Bloodmeals for Human DNA Profiling

Published online by Cambridge University Press:  19 September 2011

David O. Odongo*
Affiliation:
Department of Zoology, University of Nairobi, P.O. Box 30197 Nairobi, Kenya
Lucy W. Irungu
Affiliation:
Department of Zoology, University of Nairobi, P.O. Box 30197 Nairobi, Kenya
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Abstract

A simple method for storing mosquito bloodmeal samples, which permits extraction and detection of human DNA after polymerase chain reaction (PCR) amplification of target DNA sequences, was tested. Abdomens of bloodfed field-collected Anopheles gambiae s.l. and An. funestus mosquitoes (Diptera: Culicidae) were directly expressed onto filter paper, air-dried and stored at room temperature. DNA was extracted and amplified at human hypervariable loci TC11, VWA and D1S80. The amplified products were separated using polyacrylamide gel electrophoresis, visualised by silver staining, and results compared with those from mosquitoes that had been preserved in liquid nitrogen. DNA from blooded abdomens stored on dried filter papers could be amplified with greater than 95 % success for any locus, storage temperature, mosquito species or storage duration. Collection and drying of mosquito bloodmeals directly onto filter paper appears to be a more convenient method for sample transportation and storage than the conventional method involving cryopreservation.

Type
Research Articles
Copyright
Copyright © ICIPE 2002

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References

REFERENCES

Allen, R.C., Graves, G. and Budowle, B. (1989) Polymerase chain reaction amplification products separated on rehydratable polyacrylamide gels and stained with silver. Biotechniques 7, 736744.Google ScholarPubMed
Barker, R.H., Banchongaksorn, T., Courval, J-M., Suwonkerd, W., Rimwungtragoon, K. and Wirth, D. (1992) A simple method to detect Plasmodium falciparum directly from blood samples using polymerase chain reaction. Am. J. Trop. Med. Hyg. 46, 416426.CrossRefGoogle ScholarPubMed
Boreham, P.F.L. (1975) Some application of blood meal identification in relation to the epidemiology of vector-borne diseases, J. Trop. Med. Hyg. 78, 8291.Google Scholar
Boreham, P.F.L., Chandler, K.A. and Jolly, J. (1978) The incidence of mosquitoes feeding on mothers and babies at Kisumu, Kenya, J. Trop. Med. Hyg. 81, 6367.Google ScholarPubMed
Boreham, P.F.L. and Garret-Jones, C. (1973) Prevalence of mixed blood meals and double feeding into a malaria vector. Bull. World Hlth. Org. 46, 606614.Google Scholar
Budowle, B., Chakraborty, R., Giutsi, A.M., Eisenberg, A.J., and Allen, R.C. (1991) Analysis of the VNTR locus DS180 by the polymerase chain reaction followed by high-resolution PAGE. Am. J. Hum. Genet. 48, 137144.Google Scholar
Byran, J.H. and Smalley, M.E. (1978) The use of ABO blood groups as markers for mosquito biting studies. Trans. Roy. Soc. Trop. Med. Hyg. 72, 357360.CrossRefGoogle Scholar
Coulson, R.M.R., Curtis, C.F., Ready, P.D., Hill, N. and Smith, D.F. (1990) Amplification and analysis of human DNA in mosquito blood meals. Med.Vet. Entomol. 4, 357366.CrossRefGoogle Scholar
Gokool, S., Smith, D.F. and Curtis, C.F. (1992) The use of PCR to help quantify the protection provide by impregnated bednets. Parasit. Today. 8, 347350.CrossRefGoogle ScholarPubMed
Houba, V. (1988) Handling, preservation, storage and transportation of biological materials, pp. 18131818. In Malaria: Principles and Practice of Malariology (Edited by Wernsdorfer, W.H. and McGregor, I.). Churchill Livingstone Publishers, Edinburgh, London, Melbourne, New York.Google Scholar
Long, G.W., Fries, L., George, H.W. and Hoffman, S.L. (1995) Polymerase chain reaction amplified from P. falciparum on dried spots. Am. J. Trop. Med. Hyg. 52, 344346.CrossRefGoogle Scholar
Murray, J. C. and Murray, V. (1994) Rapid silver staining and recovery of PCR products separated on polyacrylamide gels. Biotechniques 17, 15.Google Scholar
Regine, E.W., Jean-Pierre, C., Mcnerney, R. and Wilson, S.M. (1996) Cotton wool swabs provide a convenient medium for the collection, transport and storage of sputum for the subsequent molecular investigation of Mycobacterium tuberculosis. Trans. Roy. Soc. Trop. Med. Hyg. 90, 256257.Google Scholar
Reynolds, R. and Sensabaugh, G. (1991) Analysis of genetic markers in forensic DNA samples using polymerase chain reaction. Anal. Chem. 63, 215.CrossRefGoogle ScholarPubMed
Sanjantila, A. and Budowle, B. (1991) Identification of individuals with DNA testing. Ann. Med. 23, 637642.CrossRefGoogle Scholar
Walsh, D.J., Amy, M.S., Corey, C., Robin, B.S., Cotton, R.W., Forman, L., Herrin, G.L., Word, C.J. and Garner, D.D. (1992) Isolation of DNA from saliva and forensic science samples containing saliva, J. Forensic Sci. 37, 387395.CrossRefGoogle ScholarPubMed
Walsh, P.S., Metzger, D.A. and Higushi, R. (1991) Chelex R-100 as a medium for simple extraction of DNA for PCR-based typing from forensic materials. Biotechniques 10, 506513.Google Scholar
Weitz, B. (1956) Identification of blood meals of blood sucking arthropods. Bull. World Hlth. Org. 15, 473490.Google ScholarPubMed