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Lipophorin of the larval stalk borer, Busseola Fusca: Purification and properties

Published online by Cambridge University Press:  19 September 2011

Dorington O. Ogoyi ,
Ellie O. Osir  and
James O. Ochanda
Dorington O. Ogoyi
Affiliation:
The International Centre of Insect Physiology and Ecology P. O. Box 30772, Nairobi, Kenya
Ellie O. Osir*
Affiliation:
The International Centre of Insect Physiology and Ecology P. O. Box 30772, Nairobi, Kenya
James O. Ochanda
Affiliation:
Department of Biochemistry, University of Nairobi, P. O. Box 30197, Nairobi, Kenya
*
* To whom correspondence should be addressed
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Abstract

Lipophorin was isolated from the stalk borer, Busseolafusca, larvae by ultracentrifugation in a KBr density-gradient. The lipophorin (M, ˜ 700,000) is a high density lipoprotein (density = 1.13 g/ml) composed of 46% lipids and 4% carbohydrates. It consists of two apoproteins, apolipophorin-I (apo Lp-I, Mr ˜210,000) and apolipophorin-II (apo Lp-II, Mr ˜78,000). Both apoproteins are glycosylated as shown by periodate Schiff reagent staining and binding to the lectin, concanavalin A. Amino acid composition analysis of lipophorin showed predominance of aspartate (13%), glutamate (9%) and glycine (9%).

In an immunoblotting experiment, both apoproteins showed immunoreactivity with antiserum to B, fusca lipophorin and no cross-reactivity occurred with any other haemolvmph proteins. Immunodiffusion studies with the same antibodies showed cross-reactivity with haemolymph derived from other Lepidoptera and not from Orthoptera, Dictyoptera or Diptera.

Résumé

Une lipophorine a été isolée des larves de Busseola fusca, par ultracentrifugation avec gradient de densité. La lipophorine dont le poids moléculaire est d'environ ˜700,000 est une lipoprotéine de densité élevée (1.13 mg/ml) composée de 46% de lipides et de 4% de glucides. La lipophorine comporte 2 apoprotéines: l'apolipophorine-I (apo Lp-I, de poids moleculaire environ ˜210,000) et l'apolipophorine-II (apo Lp-II, de poids moléculaire ˜78,000). Les deux apoprotéines sont glycosylées comme le démontre la coloration au réactif du périodate de Schiff (PAS). La présence de manose comportant des chaines d'oligosaccharides a été établie par la réaction d'attachement de la lipophorine à la concanavaline-A sur colonne de sépharose. L'analyse de la composition en acides aminés de la lipophorine montre une prédominance d'aspartate (13%), de glutamate (9%) et de glycine (9%). Au cours de tests d'immunoblotting, les antisera des deux apoprotéines de la lipophorine ont réagi avec les anticorps. Nos résultats suggèrent également que les lipophorines d'insectes du même ordre développeraient une cross réaction.

Keywords

Busseola fuscalipophorinphysico-chemical propertiesimmunological properties
Type
Research Articles
Information
International Journal of Tropical Insect Science , Volume 14 , Issue 2 , April 1993 , pp. 167 - 172
Copyright
Copyright © ICIPE 1993

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