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Microorganisms associated with tsetse in nature: Preliminary results on isolation, identification and pathogenicity

Published online by Cambridge University Press:  19 September 2011

Godwin P. Kaaya  and
Matilda A. Okech
Godwin P. Kaaya
Affiliation:
The International Centre of Insect Physiology and Ecology (ICIPE), P. O. Box 30772, Nairobi, Kenya
Matilda A. Okech
Affiliation:
The International Centre of Insect Physiology and Ecology (ICIPE), P. O. Box 30772, Nairobi, Kenya
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Abstract

Six genera of bacteria: Serratia, Providencia, Enterobacter, Aeromonas, Cedecea and Bacillus were isolated from field-collected G. pallidipes. Four genera: Serratia, Providencia, Enterobacter and Aeromonas were isolated from insectary-reared Glossina morsitans morsitans. All bacteria except, Bacillus spp. and Providencia alcalifaciens were pathogenic for teneral G. m. morsitans. Bacteria isolated from insectary-reared tsetse were more pathogenic than those isolated from the field. Fungi of three genera were also isolated from field-collected adult G. pallidipes, Penicillium, Aspergillus and Fusarium. Five genera, Aspergillus, Penicillium, Rhizopus, Mucor and Trichoderma were isolated from field-collected G. pallidipes pupae. The fungi, however, were only mildly pathogenic for adult tsetse and were not pathogenic for pupae.

Résumé

Six genres de bactéries: Serratia, Providencia, Enterobacter, Aeromonas, Cedecea et Bacillus ont été isolés à partir des mouches tsé-tsé adultes G. pallidipes récoltées sur le terrain. Quatre genres: Serratia, Providencia, Enterobacter et Aeromonas étaient isolés à partir de G. m. morsitans élevés à l'insectarium. Les testes de pathogénicité ont révelé que la pluspart des bactéries, à l'exception de Bacillus spp et Providencia alcalifaciens, étaient très pathogènes aux jeunes mouches tsé-tsé. Les bactéries isolées à partir des mouches tsé-tsé élevées à l'insectarium étaient très pathogènes que celles isolées des mouches tsé-tsé récoltées sur le terrain. Trois genres de champignons ont été isoles à partir des adultes de G. pallidipes récoltés sur le terrain. Ce sont Pénicillium, Aspergillus et Fusarium. Cinq genres: Aspergillus, Penicillium, Rhizopus, Mucor et Trichoderma été isolés a partir des pupes de G. pallidipes récoltées sur le terrain. Toutefois, les champignons ont montré une très faible pathogènicité aux mouches tsé-tsé adultes et étaient non pathogèns aux pupes.

Keywords

Glossina morsitans morsitansG. pallidipesSerratiaProvidenciaEnterobacterCedeceaBacillusPenicilliumAspergillusFusariumRhizopusMucorTrichodermapathogenicityGlossina morsitans morsitansG. pallidipesSerratiaProvidenciaEnterobacterCedeceaBacillusPenicilliumAspergillusFusariumRhizopusMucorTrichodermapathogénicité
Type
Research Article
Information
International Journal of Tropical Insect Science , Volume 11 , Issue 3 , June 1990 , pp. 443 - 448
Copyright
Copyright © ICIPE 1990

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