International Journal of Tropical Insect Science

Table of Contents - 1981 - Volume 2, Issue 04  

Cambridge Journals Online - CUP Full-Text Page
International Journal of Tropical Insect Science (1981), 2:213-217 Cambridge University Press
Copyright © ICIPE 1981
doi:10.1017/S1742758400001077

Research Article

Cuticular lesions: a non-infectious integumental disease of Glossina species

M. O. Odindoa1, D. A. Turnera2, W. A. Otienoa1 and G. P. Kaayaa2

a1 ICIPE, Coastal Field Station, P.O. Box 80804, Mombasa, Kenya
a2 ICIPE, P.O. Box 30772, Nairobi, Kenya
Article author query
odindo mo [PubMed]  [Google Scholar]
turner da [PubMed]  [Google Scholar]
otieno wa [PubMed]  [Google Scholar]
kaaya gp [PubMed]  [Google Scholar]

Abstract

Purified suspensions of macerated cuticular lesions from the insectary-reared tsetse Glossina morsitans morsitans were applied on laboratory-reared newly emerged teneral tsetse by three pathways: topical application on the ventral abdominal integument, oral microfeeding, or microinjection into the haemocoel. A second group of flies were subjected to teratological wounding pin-stabs, forcep-pinch and shallow blade-slits, all in attempt to induce cuticular lesions. Samples of tsetse were examined every week for 11 weeks. Application of cuticular lesion suspensions did not result in lesion formation, but three types of lesions were formed due to physical damage induced artificially on the integument.

Total haemocyte counts (THCs) and differential haemocyte counts (DHCs) were also determined for tsetse with, and without, lesions. THCs were significantly higher (P < 0.01) in tsetse with cuticular lesions than in those without lesions. DHCs showed a high number of large spindle-shaped cells and thrombocytoids in the flies with lesions (P < 0.05).

(Received July 06 1981)

Key WordsGlossina morsitans morsitans; cuticular lesions; non-infectious disease; teratology; total haemocyte count; differential haemocyte count; wound healing process

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