Epidemiology and Infection

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Epidemiology and Infection (2010), 138:525-533 Cambridge University Press
Copyright © Cambridge University Press 2009
doi:10.1017/S0950268809990823

Original Papers

Gastro-intestinal pathogens

A new multiplex PCR for differential identification of Shigella flexneri and Shigella sonnei and detection of Shigella virulence determinants


M. J. FARFÁNa1, T. A. GARAYa2, C. A. PRADOa2, I. FILLIOLa3, M. T. ULLOAa2 and C. S. TOROa2 c1

a1 Departamento de Pediatría, Hospital Dr Luis Calvo Mackenna, Facultad de Medicina, Universidad de Chile, Santiago, Chile
a2 Programa de Microbiología y Micología, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Santiago, Chile
a3 Centre National de Référence Escherichia coli et Shigella, Laboratoire des Bactéries Pathogènes Entériques, Institut Pasteur, Paris, France
Article author query
farfán mj [PubMed]  [Google Scholar]
garay ta [PubMed]  [Google Scholar]
prado ca [PubMed]  [Google Scholar]
filliol i [PubMed]  [Google Scholar]
ulloa mt [PubMed]  [Google Scholar]
toro cs [PubMed]  [Google Scholar]

SUMMARY

Most of the multiplex PCR (mPCR) used to identify Shigella do not discriminate between Shigella species or serotypes. We designed a mPCR to differentiate between S. flexneri and S. sonnei strains based on the detection of markers associated with the she pathogenicity island described in Shigella. In addition, specific primers were included to detect the Shigella virulence determinants ShET-1 and ShET-2 enterotoxin genes. The analysis of 304 Shigella strains from Chile and 79 Shigella strains from other geographic locations indicated that the mPCR described here detected all Shigella species and specifically differentiated S. flexneri and S. sonnei. The technique was sensitive, reproducible, specific and simple to perform, providing a new tool with the potential to be employed for epidemiological and diagnostic purposes.

(Accepted August 18 2009)

(Online publication September 18 2009)

Key Words:Shigella; multiplex PCR; she pathogenicity island; ShET-1; ShET-2

Correspondence:

c1 Author for correspondence: Dr C. S. Toro, Programa de Microbiología y Micología, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Independencia 1027, Independencia, Santiago, Chile. (Email: cetoro@med.uchile.cl)


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