British Journal of Nutrition

Cambridge Journals Online - CUP Full-Text Page
British Journal of Nutrition (2010), 103:502-512 Cambridge University Press
Copyright © The Authors 2009
doi:10.1017/S0007114509991978

Full Papers

Metabolism and Metabolic Studies

The protective role of amla (Emblica officinalis Gaertn.) against fructose-induced metabolic syndrome in a rat model


Hyun Young Kima1a2, Tsutomu Okuboa3, Lekh Raj Junejaa3 and Takako Yokozawaa2 c1

a1 Department of Food Science, Jinju National University, 150 Chilamdong, Jinju 660-758, Korea
a2 Institute of Natural Medicine, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan
a3 Bio-nutrition Division, Taiyo Kagaku Co. Ltd, 1–3 Takaramachi, Yokkaichi 510-0844, Japan
Article author query
kim hy [PubMed]  [Google Scholar]
okubo t [PubMed]  [Google Scholar]
juneja lr [PubMed]  [Google Scholar]
yokozawa t [PubMed]  [Google Scholar]

Abstract

We investigated the effects of amla (Emblica officinalis Gaertn.) on fructose-induced metabolic syndrome using a rat model. Male Wistar rats were fed a high-fructose (65 %) diet or standard chow for 1 week, and treated with an ethyl acetate (EtOAc) extract of amla, a polyphenol-rich fraction, at 10 or 20 mg/kg body weight per d, or vehicle, for 2 weeks. Serum glucose, TAG, total cholesterol and blood pressure levels of the high-fructose diet-fed rats were increased compared with those of the normal rats (P < 0·001). However, the EtOAc extract of amla ameliorated the high fructose-induced metabolic syndrome, including hypertriacylglycerolaemia and hypercholesterolaemia. Also, the elevated levels of hepatic TAG and total cholesterol in rats given the high-fructose diet were significantly reduced by 33·8 and 24·6 %, respectively (P < 0·001), on the administration of the EtOAc extract of amla at the dose of 20 mg/kg with the regulation of sterol regulatory element-binding protein (SREBP)-1 expression. The protein levels of PPARα and SREBP-2 were not affected by the feeding of the high-fructose diet or EtOAc extract of amla. In addition, oral administration of the amla extract at the dose of 20 mg/kg significantly inhibited the increased serum and hepatic mitochondrial thiobarbituric acid-reactive substance levels (21·1 and 43·1 %, respectively; P < 0·001). Furthermore, the amla extract inhibited the increase of cyclo-oxygenase-2 with the regulation of NF-κB and bcl-2 proteins in the liver, while the elevated expression level of bax was significantly decreased by 8·5 and 10·2 % at the doses of 10 and 20 mg/kg body weight per d, respectively. These findings suggest that fructose-induced metabolic syndrome is attenuated by the polyphenol-rich fraction of amla.

(Received April 17 2009)

(Revised August 10 2009)

(Accepted August 11 2009)

(Online publication November 02 2009)

Key Words:Amla; High-fructose diet; Metabolic syndrome; Sterol regulatory element-binding protein-1; NF-κB

Correspondence:

c1 Corresponding author: Dr Takako Yokozawa, fax +81 76 434 5068, email yokozawa@inm.u-toyama.ac.jp

Footnotes

Abbreviations: BW, body weight; COX-2, cyclo-oxygenase-2; EtOAc, ethyl acetate; IDL, intermediate-density lipoprotein; I-κBα, inhibitor binding protein κB-α; iNOS, inducible NO synthase; SREBP, sterol regulatory element-binding protein; TBA, thiobarbituric acid; Tris, 2-amino-2-hydroxymethyl-1,3-propanediol


0Comments