a1 College of Agronomy and Biotechnology, Southwest University, Chongqing 400716, China
In exploring an effective and reliable karyotyping method in Brassica crop plants, Brassica oleracea was successfully karyotyped using genomic in situ hybridization (GISH). B. oleracea genomic DNA was labelled as probe using DIG-high prime mix kit, with B. rapa genomic DNA acting as blocking agent. Specific fluorescent signals were detected on each pair of homologous chromosomes, and nine pairs of chromosomes of B. oleracea were clearly identified according to the signal characteristics. A practical and accurate method for conducting karyotyping of small chromosomes has been demonstrated.
(Received June 03 2008)
(Accepted July 07 2008)
First published in Journal of Agricultural Biotechnology 2009, 17(3): 504–509