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Sugarbeet seed priming: effects of priming conditions on germination, solubilization of 11-S globulin and accumulation of LEA proteins

Published online by Cambridge University Press:  22 February 2007

Isabelle Capron ,
Françoise Corbineau ,
François Dacher ,
Claudette Job ,
Daniel Côme  and
Dominique Job
Isabelle Capron
Affiliation:
Laboratoire mixte CNRS/INRA/Aventis (UMR1932), Aventis CropScience, 14-20 rue Pierre Baizet, 69263 Lyon cedex 9, France
Françoise Corbineau
Affiliation:
Physiologie Végétale Appliquée, Université Pierre et Marie Curie, Tour 53, 1er étage, 4 place Jussieu, 75252 Paris cedex 05, France
François Dacher
Affiliation:
Physiologie Végétale Appliquée, Université Pierre et Marie Curie, Tour 53, 1er étage, 4 place Jussieu, 75252 Paris cedex 05, France
Claudette Job
Affiliation:
Laboratoire mixte CNRS/INRA/Aventis (UMR1932), Aventis CropScience, 14-20 rue Pierre Baizet, 69263 Lyon cedex 9, France
Daniel Côme
Affiliation:
Physiologie Végétale Appliquée, Université Pierre et Marie Curie, Tour 53, 1er étage, 4 place Jussieu, 75252 Paris cedex 05, France
Dominique Job*
Affiliation:
Laboratoire mixte CNRS/INRA/Aventis (UMR1932), Aventis CropScience, 14-20 rue Pierre Baizet, 69263 Lyon cedex 9, France
*
*Correspondence Tel: + 33 4 72 85 21 79 Fax: +33 4 72 85 22 97 Email: dominique.job@aventis.com
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Abstract

The relationship between initial mobilization of 11-S globulin storage protein (leading to solubilization of its B-subunit) and germination performance after priming was studied in sugarbeet (Beta vulgaris L.) seeds. Priming was conducted for 2 d either in water (hydropriming) or in –2.0 MPa polyethylene glycol-8000 (osmopriming), at various temperatures ranging from 5 to 40°C and oxygen concentrations in the atmosphere ranging from 0 to 21%. For both types of pre-treatments, the range of temperatures and the concentrations of oxygen which were effective in priming were very similar to those which allowed solubilization of the B-subunit of 11-S globulin, supporting the robustness of this protein marker for optimization of sugarbeet seed priming. Furthermore, the temperature and oxygen dependence of priming efficiency closely paralleled that for germination of the untreated seeds, reinforcing the finding that the beneficial effect of priming corresponded to the advancement of germination sensu stricto (i.e. phase II of the germination process). For priming times longer than 2 d, particularly for osmopriming, there was a very dramatic decrease in germination of the treated seeds. For instance, following a 14 d osmopriming at 25°C as much as 60% of the pre-treated seed population failed to germinate when transferred to water. This loss in germination performance quite closely paralleled degradation of LEA (late embryogenesis abundant) proteins, notably a heat-stable seed-specific protein of about 60 kDa and a seed-specific biotinylated LEA protein.

Keywords

seed priming11-S globulinsLEA proteinsbiotinylated proteinssugarbeetBeta vulgaris
Type
Research Article
Information
Seed Science Research , Volume 10 , Issue 3 , September 2000 , pp. 243 - 254
Copyright
Copyright © Cambridge University Press 2000

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