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Pre-harvest stress cracks in maize (Zea mays L.) kernels as characterized by visual, X-ray and low temperature scanning electron microscopical analysis: effect on kernel quality

Published online by Cambridge University Press:  22 February 2007

Maria Laene Moreira de Carvalho ,
Adriaan C. van Aelst ,
Jan Walter van Eck  and
Folkert A. Hoekstra
Maria Laene Moreira de Carvalho*
Affiliation:
Department of Agriculture, Universidade Federal de Lavras, P.O.Box 37, 37750–000 Lavras, MG, Brazil Laboratories of Plant Physiology, Wageningen Agricultural University, Arboretumlaan 4, 6703 BD Wageningen, The, Netherlands
Adriaan C. van Aelst*
Affiliation:
Plant Cytology and Morphology, Wageningen Agricultural University, Arboretumlaan 4, 6703 BD Wageningen, The, Netherlands
Jan Walter van Eck*
Affiliation:
DLO-Centre for Plant Breeding and Reproduction Research, P.O. Box 16, 6700 AA Wageningen, The, Netherlands
Folkert A. Hoekstra*
Affiliation:
Laboratories of Plant Physiology, Wageningen Agricultural University, Arboretumlaan 4, 6703 BD Wageningen, The, Netherlands
*
Corresponding author: Folkert A. Hoekstra, Laboratory of Plant Physiology, Agricultural University, Arboretumlaan 4, NL - 6703 BD Wageningen, The Netherlands Fax: +31 317 484740 Email: folkert.hoekstra@algem.pf.wau.nl
Corresponding author: Folkert A. Hoekstra, Laboratory of Plant Physiology, Agricultural University, Arboretumlaan 4, NL - 6703 BD Wageningen, The Netherlands Fax: +31 317 484740 Email: folkert.hoekstra@algem.pf.wau.nl
Corresponding author: Folkert A. Hoekstra, Laboratory of Plant Physiology, Agricultural University, Arboretumlaan 4, NL - 6703 BD Wageningen, The Netherlands Fax: +31 317 484740 Email: folkert.hoekstra@algem.pf.wau.nl
Corresponding author: Folkert A. Hoekstra, Laboratory of Plant Physiology, Agricultural University, Arboretumlaan 4, NL - 6703 BD Wageningen, The Netherlands Fax: +31 317 484740 Email: folkert.hoekstra@algem.pf.wau.nl
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Abstract

Internal cracks caused by high temperature or excessive moisture during maize (Zea mays L.) kernel development were characterized, and their effects on kernel quality were assessed. Pre-harvest stress cracks are often located near the middle of the kernel along the embryo axis, but they were also detected in other positions, irrespective of the shape of the kernel. X-ray analysis enabled visualisation of stress cracks that are invisible to the human eye and, therefore, gave a better estimate of the percentage of cracks. However, low temperature scanning electron microscopy of the surface of milled kernels revealed small cracks not noticed by visual or X-ray inspection. All kernels tested in this way had a crack of some sort in the endosperm tissue. Cracks were also frequent in the scutellum, but rare in the embryo axis. Endosperm cracks followed the boundary of the starch granules, but did not extend into the pericarp tissue. In contrast to external cracks caused by mechanical impact, pre-harvest internal stress cracks generally are not detrimental to germination and vigour. However, if the crack is located inside or perpendicular to the embryo axis, it may affect the quality of the kernel, probably by impeding nutrient translocation to the embryo.

Keywords

cracksgerminationkernelscanning electron microscopyvigourX-ray analysisZea maysL
Type
Research Article
Information
Seed Science Research , Volume 9 , Issue 3 , March 1999 , pp. 227 - 236
Copyright
Copyright © Cambridge University Press 1999

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References

Adegbuyi, E. and Burris, J.S. (1988) Field criteria used in determining the vigor of seed corn (Zea mays L.) as influenced by drying injury. Journal of Agronomy and Crop Science 161, 171–177.CrossRefGoogle Scholar
Association of Official Seed Analysts (AOSA) (1983) Seed vigor testing handbook. East Lansing, AOSA.Google Scholar
Burris, J.S., Peterson, J.M., Perdomo, A.J. and Feng, D.S. (1997) Morphological and physiological changes associated with desiccation in maize embryos. pp 103–111 in Ellis, R.H.; Black, M.; Murdoch, A.J.; Hong, T.D. (Eds) Basic and applied aspects of seed biology. Dordrecht, Netherlands, Kluwer Academic Publishers.CrossRefGoogle Scholar
Carvalho, M.L.M., Oliveira, J.A. and Vieira, M.G.G.C.V. (1994) Uso de corantes na determinação de danos em milho [Dye uses on seed corn damage determination]. pp 220–221in Anais do XX Congresso Nacional de milho e sorgo. Goiânia, Brazil, Associac. Brasileira de milho e sorgo.Google Scholar
Cicero, S.M., Van der Heijden, G.W.A.M., Van der Burg, W.J. and Bino, R.J. (1996) Avaliação de danos mecanicos em sementes de milho por meio de analise de imagens [Evaluation of mechanical damage in seeds of maize by image analysis]. p 229in Congresso Nacional de Milho e Sorg XXI, 1996, Londrina Resumos. Londrina, Brasil, Instituto Agronomico do Parana (IAPAR).Google Scholar
Chowdhury, M.H. and Buchele, W.F. (1976) Development of a numerical damage index for critical evaluation of mechanical damage of corn. Transactions of the ASAE 19, 428–432.CrossRefGoogle Scholar
Chowdhury, M.H. (1977) Methods for determining grain damage (mechanical). pp 216–228in Marley, S.J.; Buchele, W.F. (Eds.) Proceedings of the fourth international conference on mechanisation of field experiments. Ames, Iowa State University Press.Google Scholar
Dias, M.C.L. and Barros, A. (1995) Avaliação da qualidade de sementes de milho [ Evaluation of seed corn quality]. Circular 88, Londrina, Brasil, Instituto Agronomico do Parana (IAPAR).Google Scholar
Escasinas, A.B. and Hill, M.J. (1988) Stress cracking in maize (Zea mays L.) seeds. Annals of Tropical Research 10, 182–192.Google Scholar
Girardin, P., Chavagnat, A. and Bockstaller, C. (1993) Détermination des caractéristiques des semences de maïs grâce ` la radiographie aux rayons X. [Determination of seed characteriztics of Maize (Zea mays L.) by use of X-ray radiography]. Seed Science and Technology 21, 545–551.Google Scholar
Golovina, E.A., Tikhonov, A.N. and Hoekstra, F.A. (1997) An electron paramagnetic resonance spin probe study of membrane permeability changes with seed aging. Plant Physiology 114, 383–389.CrossRefGoogle ScholarPubMed
Gonçalves, C.A.R. (1981) Efeitos de métodos de colheita e debulha de sementes sobre a germinação e produção de milho [Effect of harvest and thresh methods on corn germination and production]. PhD Thesis, Escola Superior de Agricultura Luiz de Queiroz, University of São Paulo, Piracicaba.Google Scholar
Gunasekaran, S., Deshpande, S.S., Paulsen, M.R. and Shove, G.C. (1985) Size characterization of stress cracks in corn kernels. Transactions of the ASAE 28, 1668–1672.CrossRefGoogle Scholar
Gunasekaran, S. and Paulsen, M.R. (1985) Breakage resistance of corn as a function of drying rates. Transactions of the ASAE 28, 2071–2076.CrossRefGoogle Scholar
Herter, U. and Burris, J.S. (1989) Evaluating drying injury on corn seed with a conductivity test. Seed Science and Technology 17, 625–638.Google Scholar
MARA (1992) Rules of seed analysis [Regras para Analises de Sementes]. Brasilia, Brasil, Ministerio da Agricultura e Reforma Agraria (MARA).Google Scholar
Naplava, V. and Weingartman, H. (1994) Stress cracks during seed corn drying. Zemedelska Technika 40, 3–14.Google Scholar
Peterson, J.M., Perdomo, J.A. and Burris, J.S. (1995) Influence of kernel position, mechanical damage and controlled deterioration on estimates of hybrid maize seed quality. Seed Science and Technology 23, 647–657.Google Scholar
Plett, S. (1994) Corn kernel breakage as a function of grain moisture at harvest in a prairie environment. Canadian Journal of Plant Science 74, 543–544.CrossRefGoogle Scholar
Reid, J.F., Kim, C. and Paulsen, M.R. (1991) Computer vision sensing of stress cracks in corn kernels. Transactions of the ASAE 34, 2236–2244.CrossRefGoogle Scholar
Sato, O. and Cicero, S.M. (1992) Seleção de espigas e debulha das sementes de milho (Zea mays L.). Efeitos sobre a qualidade fisica e infestação por insetos [Ear selection and thresh of corn seed. Effects on physical quality and infestation by insects]. Scientia Agricola 49, 93–101.CrossRefGoogle Scholar
Sharma, A.D., Tolley, H.D. and Kunze, O.R. (1979) A two components drying model related to the fissuring in rough rice. ASAE Paper 79, 3550, St Joseph, ASEA.Google Scholar
Snedecor, G.W. and Cochran, W.G. (1989) Statistical methods. 8th edition, Ames, Iowa State University Press.Google Scholar
Vyn, T.J. and Moes, J. (1988) Breakage susceptibility of corn kernels in relation to crop management under long growing season conditions. Agronomy Journal 80, 915–920.CrossRefGoogle Scholar
Zaleski, A., Gladysz, A. and Kowalska, I. (1992) Seed testing with the use of X-rays. Prace Instytutu-Bada Wazego-Iesnictwa Poland 724, 69–93.Google Scholar