Hostname: page-component-76fb5796d-25wd4 Total loading time: 0 Render date: 2024-04-25T14:46:41.853Z Has data issue: false hasContentIssue false
  • English
  • Français

Is the sperm centrosome to blame for the complex polyploid chromosome patterns observed in cleavage stage embryos from an OAT patient?

Published online by Cambridge University Press:  01 February 2007

K. Chatzimeletiou ,
A. J. Rutherford ,
D. K. Griffin  and
A. H. Handyside
K. Chatzimeletiou*
Affiliation:
The London Bridge Fertility, Gynaecology and Genetics Centre, 1 St Thomas Street, London SE1 9RY, UK. School of Biology, University of Leeds, Leeds LS2 9JT, UK.
A. J. Rutherford
Affiliation:
Assisted Conception Unit, Clarendon Wing, Leeds General Infirmary, Leeds LS2 9NS, UK.
D. K. Griffin
Affiliation:
Dept of Biosciences, University of Kent, Canterbury, CT2 7NJ, UK.
A. H. Handyside
Affiliation:
The London Bridge Fertility, Gynaecology and Genetics Centre, 1 St Thomas Street, London SE1 9RY, UK. School of Biology, University of Leeds, Leeds LS2 9JT, UK.
*
All correspondence to: Katerina Chatzimeletiou, The London Bridge Fertility, Gynaecology and Genetics Centre, 1 St Thomas Street, London, SE1 9RY, UK. Tel: +44 0207 403 3363. e-mail: katerinachatzime@hotmail.com
Get access Rights & Permissions [Opens in a new window]

Summary

Oligoasthenoteratozoospermia (OAT) is defined by a combined low count < 20 × 106 sperm/ml, poor motility < 50 % forward progression or < 25 % rapid linear progression and abnormal morphology (5–8 % normal using Kruger strict criteria) and has been associated with increased levels of sperm aneuploidy. Here we report on the cytogenetic findings from three ‘spare’ embryos from a couple that were referred for ICSI because of OAT. The embryos were processed for sequential FISH in three hybridization rounds using probes for chromosomes 3, 7, 9, 13, 17, 18, 21, X and Y. Molecular cytogenetic analysis of nine chromosomes revealed that all three embryos were female polyploid. One of them was uniformly tetraploid for all chromosomes tested, while the remaining two embryos showed evidence of abnormal postzygotic segregation of chromosomes, causing the derivative blastomeres to have uneven chromosomal constitution. In one of them in particular, the non-disjoining chromosomes showed preferential segregation to the same pole, rather than randomly moving towards either pole, suggesting an abnormal spindle and causing the derivative blastomeres to have significantly uneven chromosomal constitutions. The possible scenarios leading to polyploidy and chromosomal imbalance through cytokinetic failure and subsequent abnormal centrosomal distribution are outlined.

Keywords

OligoasthenoteratozoospermiaPolyploidyPostzygotic non-disjunctionSequential FISHSpindle defect
Type
Research Article
Information
Zygote , Volume 15 , Issue 1 , February 2007 , pp. 81 - 90
Copyright
Copyright © Cambridge University Press 2007

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Calogero, A.E., De Palma, A., Grazioso, C., Barone, N., Burrello, N., Palermo, I., Gulisano, A., Pafumi, C. & D'Agata, R. (2001 a). High sperm aneuploidy rate in unselected infertile patients and its relationship with intracytoplasmic sperm injection outcome. Human Reprod. 16, 1433–9.CrossRefGoogle ScholarPubMed
Calogero, A.E., De Palma, A., Grazioso, C., Barone, N., Romeo, R., Rappazzo, G. & D’Agata, R. (2001 b). Aneuploidy rate in spermatozoa of selected men with abnormal semen parameters. Human Reprod. 16, 1172–9.CrossRefGoogle ScholarPubMed
Chatzimeletiou, K., Morrison, E.E., Panagiotidis, Y., Prapas, N., Prapas, Y., Rutherford, A.J., Grudzinskas, G. & Handyside, A.H (2005 a). Comparison of effects of zona drilling by non-contact infrared laser or acid Tyrode's on the development of human biopsied embryos as revealed by blastomere viability, cytoskeletal analysis and molecular cytogenetics. Reprod. Biomed. Online 11, 697–710.CrossRefGoogle ScholarPubMed
Chatzimeletiou, K., Morrison, E.E., Prapas, N., Prapas, Y. & Handyside, A.H. (2005 b). Spindle abnormalities in normally developing and arrested human preimplantation embryos in vitro identified by confocal laser scanning microscopy. Human Reprod. 20, 672–82.CrossRefGoogle ScholarPubMed
Griffin, D.K. & Finch, K. (2005). The genetic and cytogenetic basis of male infertility. Human Fertility 8, 19–26.CrossRefGoogle ScholarPubMed
In't Veld, P.A., Broekmans, F.J., de France, H.F., Pearson, P.L., Pieters, M.H. & van Kooij, R.J. (1997). Intracytoplasmic sperm injection (ICSI) and chromosomally abnormal spermatozoa. Human Reprod. 12, 752–4.CrossRefGoogle ScholarPubMed
Pang, M.G., Hoegerman, S.F., Cuticchia, A.J., Moon, S.Y., Doncel, G.F., Acosta, A.A. & Kearns, W.G. (1999). Detection of aneuploidy for chromosomes 4, 6, 7, 8, 9, 10, 11, 12, 13, 17, 18, 21, X and Y by fluorescence in-situ hybridization in spermatozoa from nine patients with oligoasthenoteratozoospermia undergoing intracytoplasmic sperm injection. Human Reprod. 14, 1266–73.CrossRefGoogle ScholarPubMed
Pfeffer, J., Pang, M.G., Hoegerman, S.F., Osgood, C.J., Stacey, M.W., Mayer, J., Oehninger, S. & Kearns, W.G. (1999). Aneuploidy frequencies in semen fractions from ten oligoasthenoteratozoospermic patients donating sperm for intracytoplasmic sperm injection. Fertility & Sterility 72, 472–8.CrossRefGoogle ScholarPubMed
Tempest, H.G. & Griffin, D.K. (2004). The relationship between male infertility and increased levels of sperm disomy. Cytogenet. Genome Res. 107, 83–94.CrossRefGoogle ScholarPubMed
Tesarik, J., Mendoza, C. & Greco, E. (2002). Paternal effects acting during the first cell cycle of human preimplantation development after ICSI. Human Reprod. 17, 184–9.CrossRefGoogle ScholarPubMed