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Variations in the mitochondrial cytochrome c oxidase subunit I gene indicate northward expanding populations of Culicoides imicola in Spain

Published online by Cambridge University Press:  19 February 2009

J.H. Calvo ,
C. Calvete ,
A. Martinez-Royo ,
R. Estrada ,
M.A. Miranda ,
D. Borras ,
V. Sarto I Monteys ,
N. Pages ,
J.A. Delgado  and
F. Collantes
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J.H. Calvo*
Affiliation:
Unidad de Tecnología en Producción animal, CITA-ARAID, Zaragoza, Spain
C. Calvete
Affiliation:
Unidad Sanidad Animal, CITA, Zaragoza. Spain
A. Martinez-Royo
Affiliation:
Unidad de Tecnología en Producción animal, CITA-ARAID, Zaragoza, Spain
R. Estrada
Affiliation:
Departamento de Patología Animal, Universidad de Zaragoza, Zaragoza, Spain
M.A. Miranda
Affiliation:
Laboratorio de Zoología, Universidad de las Islas Baleares, Palma de Mallorca, Spain
D. Borras
Affiliation:
Laboratorio de Zoología, Universidad de las Islas Baleares, Palma de Mallorca, Spain
V. Sarto I Monteys
Affiliation:
Unidad de Entomología. Fundación CReSA, UAB Bellaterra, Spain
N. Pages
Affiliation:
Unidad de Entomología. Fundación CReSA, UAB Bellaterra, Spain
J.A. Delgado
Affiliation:
Departamento de Zoología y Antropología Física, Universidad de Murcia, Murcia, Spain
F. Collantes
Affiliation:
Departamento de Zoología y Antropología Física, Universidad de Murcia, Murcia, Spain
J. Lucientes
Affiliation:
Departamento de Patología Animal, Universidad de Zaragoza, Zaragoza, Spain
*
*Author for correspondence Fax: +34 976716335 E-mail: jhcalvo@aragon.es
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Abstract

Culicoides imicola is the main vector for bluetongue (BT) and African horse sickness (AHS) viruses in the Mediterranean basin and in southern Europe. In this study, we analysed partial mitochondrial cytochrome c oxidase subunit I (COI) gene to characterize and confirm population expansion of Culicoides imicola across Spain. The data were analysed at two hierarchical levels to test the relationship between C. imicola haplotypes in Spain (n=215 from 58 different locations) and worldwide (n=277). We found nineteen different haplotypes within the Spanish population, including 11 new haplotypes. No matrilineal subdivision was found within the Spanish population, while western and eastern Mediterranean C. imicola populations were very structured. These findings were further supported by median networks and mismatch haplotype distributions. Median networks demonstrated that the haplotypes we observed in the western Mediterranean region were closely related with one another, creating a clear star-like phylogeny separated only by a single mutation from eastern haplotypes. The two, genetically distinct, sources of C. imicola in the Mediterranean basin, thus, were confirmed. This type of star-like population structure centred around the most frequent haplotype is best explained by rapid expansion. Furthermore, the proposed northern expansion was also supported by the statistically negative Tajima's D and Fu's Fs values, as well as predicted mismatch distributions of sudden and spatially expanding populations. Our results thus indicated that C. imicola population expansion was a rapid and recent phenomenon.

Keywords

Culicoides imicolaAfrican horse sicknessbluetonguecytochrome c oxidase Ipopulation expansionmtDNA
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 99 , Issue 6 , December 2009 , pp. 583 - 591
Copyright
Copyright © 2009 Cambridge University Press

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