Journal of Helminthology

Research Papers

Development of an immunobinding dot-blot assay as an alternative for the serodiagnosis of human cysticercosis

E. Hernández-Cruza1, J.J. González-Cabrialesa1, C. Ordaz-Pichardoa1, N.I. de la Cruz-Hernándeza2 and G.H. Flores-Gutiérreza1 c1

a1 Unidad Académica Multidisciplinaria Reynosa-Aztlán, Universidad Autónoma de Tamaulipas, Reynosa, Tamaulipas, México

a2 Facultad de Medicina Veterinaria y Zootecnia ‘Dr. Norberto Treviño Zapata’, Universidad Autónoma de Tamaulipas, Victoria, Tamaulipas, México

Abstract

An immunobinding dot-blot assay (DBA) was developed on nitrocellulose paper for the serodiagnosis of human cysticercosis, using Cysticercus cellulosae as antigen. The DBA had an immunological sensitivity of 0.08 mg of antigen protein/ml; however, it showed cross-reactions with antigens of adult Taenia solium and Echinococcus granulosus, but not with Toxoplasma gondii and Entamoeba histolytica antigens. An enzyme-linked immunosorbent assay (ELISA) was used as the gold standard for obtaining the diagnostic validity of the DBA, giving 84.61%, 100.00%, 100.00% and 97.98% for epidemiological sensitivity, epidemiological specificity and positive and negative predictive values, respectively. There were no statistical differences between the two tests (P < 0.05, kappa = 0.907). This study showed that DBA is an alternative method for the serodiagnosis of human cysticercosis.

(Accepted January 06 2009)

(Online publication March 25 2009)

Correspondence:

c1 Fax: +52 899 923 0622 E-mail: ghflores@uat.edu.mx