Genetical Research



SHORT PAPER

Estimation of microsatellite mutation rates in Drosophila melanogaster


JOSÉ FERNANDO VÁZQUEZ a1, TRINIDAD PÉREZ a1, JESÚS ALBORNOZ a1 and ANA DOMÍNGUEZ a1c1
a1 Area de Genética, Departamento de Biología Funcional, Universidad de Oviedo, 33071 Oviedo, Spain

Abstract

Microsatellite mutations were studied in a set of 175 mutation accumulation lines, all of them independently derived from a completely homozygous population of Drosophila melanogaster and maintained under strong inbreeding during 80 generations. We assayed 28 microsatellites and detected two mutations. One mutation consisted of a single addition of a dinucleotide repeat and the other was a deletion of five trinucleotide repeats. The average mutation rate was 5·1 × 10−6, in full agreement with previous estimates from two different sets of mutation accumulation lines.

(Received May 29 2000)
(Revised July 6 2000)


Correspondence:
c1 Corresponding author. e-mail: ads@sauron.quimica.uniovi.es


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