Hostname: page-component-8448b6f56d-t5pn6 Total loading time: 0 Render date: 2024-04-20T01:58:22.419Z Has data issue: false hasContentIssue false
  • English
  • Français

Bifidogenic effect and stimulation of short chain fatty acid production in human faecal slurry cultures by oligosaccharides derived from lactose and lactulose

Published online by Cambridge University Press:  18 May 2009

Alejandra Cardelle-Cobas ,
María Fernández ,
Nuria Salazar ,
Cristina Martínez-Villaluenga ,
Mar Villamiel ,
Patricia Ruas-Madiedo  and
Clara G de los Reyes-Gavilán
Alejandra Cardelle-Cobas
Affiliation:
Instituto de Fermentaciones Industriales (CSIC), Juan de la Cierva, 3, Madrid28006, Spain
María Fernández
Affiliation:
Instituto de Productos Lácteos de Asturias (CSIC), Crta. Infiesto s/n, 33300Villaviciosa (Asturias), Spain
Nuria Salazar
Affiliation:
Instituto de Productos Lácteos de Asturias (CSIC), Crta. Infiesto s/n, 33300Villaviciosa (Asturias), Spain
Cristina Martínez-Villaluenga
Affiliation:
Instituto de Fermentaciones Industriales (CSIC), Juan de la Cierva, 3, Madrid28006, Spain
Mar Villamiel*
Affiliation:
Instituto de Fermentaciones Industriales (CSIC), Juan de la Cierva, 3, Madrid28006, Spain
Patricia Ruas-Madiedo
Affiliation:
Instituto de Productos Lácteos de Asturias (CSIC), Crta. Infiesto s/n, 33300Villaviciosa (Asturias), Spain
Clara G de los Reyes-Gavilán
Affiliation:
Instituto de Productos Lácteos de Asturias (CSIC), Crta. Infiesto s/n, 33300Villaviciosa (Asturias), Spain
*
*For correspondence; e-mail: mvillamiel@ifi.csic.es
Get access Rights & Permissions [Opens in a new window]

Abstract

Bifidogenic effect and stimulation of short chain fatty acid (SCFA) production by fractions of oligosaccharides with a DP⩾3 and Gal β(1-6) linkages synthesised from lactose or lactulose by Pectinex Ultra SP-L and Lactozym 3000 L HP G were evaluated in human faecal slurries. Results were compared with those obtained for the commercial oligosaccharide mixture Vivinal®-GOS. Quantitative real-time PCR showed that all galacto-oligosaccharide (GOS) fractions stimulated slightly higher bifidobacteria growth than lactose, lactulose and Vivinal®-GOS. GOS fractions promoted the production of total SCFA and acetic acid in a similar way to Vivinal®-GOS and more than glucose, lactose and lactulose. In conclusion, oligosaccharides derived from lactose and lactulose may represent a suitable alternative to lactulose for the design of new functional food ingredients.

Keywords

Oligosaccharidesbifidogenic effectshort chain fatty acidslactoselactulose
Type
Research Article
Information
Journal of Dairy Research , Volume 76 , Issue 3 , August 2009 , pp. 317 - 325
Copyright
Copyright © Proprietors of Journal of Dairy Research 2009

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Aider, M & de Halleux, D 2007 Isomerization of lactose and lactulose production: a review. Trends in Food Science and Technology 18 356–364Google Scholar
Al-Tamimi, MAY, Palframan, RJ, Cooper, JM, Gibson, GR & Rastall, RA 2006 In vitro fermentation of sugar beet arabinan and arabino-oligosaccharides by the human gut microflora. Journal of Applied Microbiology 100 407–414CrossRefGoogle ScholarPubMed
Anthony, JC, Merriman, TN & Heimbach, JT 2006 90-Day oral (gavage) study in rats with galactooligosaccharides syrup. Food Chemistry and Toxicology 44 819–826CrossRefGoogle ScholarPubMed
Barreteau, H, Delattre, C & Michaud, P 2006 Production of oligosaccharides as promising new food additive generation. Food Technology and Biotechnology 44 323–333Google Scholar
Bouhnik, Y, Raskine, L, Simoneau, G, Vicaut, E, Neut, C, Flourie, B, Brouns, F & Bornet, FR 2004a The capacity of nondigestible carbohydrates to stimulate fecal bifidobacteria in healthy humans: a double-blind, randomized, placebo-controlled, parallel-group, dose-response relation study. American Journal of Clinical Nutrition 80 1658–1664CrossRefGoogle ScholarPubMed
Bouhnik, Y, Attar, A, Joly, FA, Riottot, M, Dyard, F & Flourie, B 2004b Lactulose ingestion increases faecal bifidobacterial counts: a randomised double-blind study in healthy humans. European Journal of Clinical Nutrition 58 462–466Google Scholar
Cardelle-Cobas, A, Villamiel, M, Olano, A & Corzo, N 2008a Study of galacto-oligosaccharide formation from lactose using Pectinex-Ultra SP-L. Journal of the Science of Food and Agriculture 88 954–961CrossRefGoogle Scholar
Cardelle-Cobas, A, Martínez-Villaluenga, C, Villamiel, M, Olano, A & Corzo, N 2008b Synthesis of oligosacchariedes derived from lactulose and Pectinex Ultra SP L. Journal of Agricultural and Food Chemistry 56 3328–3333CrossRefGoogle ScholarPubMed
Chockchaisawasdee, S, Athanasopoulos, VI, Niranjan, K & Rastall, RA 2005 Synthesis of galacto-oligosaccharides from lactose using, β-galactosidase from Kluyveromyces lactis: Studies on batch and continuous UF membrane fitted bioreactors. Biotechnology and Bioengineering 89 434–443CrossRefGoogle ScholarPubMed
Chow, J 2002 Probiotics and Prebiotics: a brief overview. Journal of Renal Nutrition 12 76–86CrossRefGoogle ScholarPubMed
Cristl, SU, Murgatroyd, PR, Gibson, GR & Cummings, JH 1992 Production metabolism and excretion of hydrogen in the large intestine. Gastroenterology 102 1269–1277CrossRefGoogle Scholar
Cummings, JH 1981 Short chain fatty acids in the human colon. Gut 22 763–779Google Scholar
De Preter, V, Vanhoutte, T, Huys, G, Swings, J, Rutgeerts, P & Verbeke, K 2008 Baseline microbiota activity and influence responses to prebiotic initial bifidobacteria counts dosing in healthy subjects. Alimentary Pharmacology and Therapeutics 27 504–513Google Scholar
Delzenne, NM 2003 Oligosaccharides: state of the art. Proceedings of the Nutrition Society 62 177–182CrossRefGoogle ScholarPubMed
Djouzi, Z & Andrieux, C 1997 Compared effects of three oligosaccharides on metabolism of intestinal microflora in rats inoculated with a human faecal flora. British Journal of Nutrition 78 313–324CrossRefGoogle ScholarPubMed
Dongowski, G, Jacobasch, G & Schmiedl, D 2005 Structural stability and prebiotic properties of resistant starch type 3 increase bile acid turnover and lower secondary bile acid formation. Journal of Agricultural and Food Chemistry 53 9257–9267CrossRefGoogle ScholarPubMed
Gibson, GR & Roberfroid, MB 1995 Dietary modulation of the human colonic microbiota: introducing the concept of prebiotics. Journal of Nutrition 125 1401–1412CrossRefGoogle ScholarPubMed
Gueimonde, M, Tölkko, S, Korpimäki, T & Salminen, S 2004 New real-time quantitative PCR procedure for quantification of bifidobacteria in human fecal samples. Applied and Environmental Microbiology 70 4165–4169CrossRefGoogle ScholarPubMed
Holt, SM, Miller-Fosmore, CM & Côté, GL 2005 Growth of various intestinal bacteria on alternansucrase-derived oligosaccharides. Letters in Applied Microbiology 40 385–390CrossRefGoogle ScholarPubMed
Hsu, CA, Lee, SL, Chou, CC 2007 Enzymatic production of galactooligosaccharides by β-galactosidase from Bifidobacterium longum BCRC 15708. Journal of Agricultural and Food Chemistry 55 2225–2230CrossRefGoogle ScholarPubMed
Kaneko, T, Kohmoto, T, Kikuchi, H, Shiota, M, Iino, H & Mitsuoka, T 1994 Effects of isomaltooligosaccharides with different degrees of polymerization on human faecal Bifidobacteria. Bioscience Biotechnology and Biochemistry 58 2288–2290Google Scholar
Kaplan, H & Hutkins, RW 2000 Fermentation of fructooligosaccharides by lactic acid bacteria and bifidobacteria. Applied and Environmental Microbiology 66 2682–2684CrossRefGoogle ScholarPubMed
Louis, P, Scott, KP, Duncan, SH & Flint, HJ 2007 Understanding the effects of diet on bacterial metabolism in the large intestine. Journal of Applied Microbiology 102 1197–1208Google Scholar
Macfarlane, S & Macfarlane, GT 2003 Regulation of short-chain fatty acid production. Proceedings of the Nutrition Society 62 67–72CrossRefGoogle ScholarPubMed
Macfarlane, S, Macfarlane, GT, Cummings, JH 2006 Review article: prebiotics in the gastrointestinal tract. Alimentary Pharmacology & Therapeutics 24 701–714Google Scholar
Mahoney, RR 1998 Galactosyl-oligosaccharide formation during lactose hydrolysis: a review. Food Chemistry 63 147–154Google Scholar
Martínez-Villaluenga, C, Cardelle-Cobas, A, Corzo, N, Olano, A & Villamiel, M 2008a Optimization of conditions for galactooligosaccharide synthesis during lactose hydrolysis by β-galactosidase from Kluyveromyces lactis (Lactozym 3000 L HP G). Food Chemistry 107 258–264CrossRefGoogle Scholar
Martínez-Villaluenga, C, Cardelle-Cobas, A, Olano, A, Corzo, N, Villamiel, M, Jimeno, ML 2008b Enzymatic synthesis and identification of two trisaccharides produced from lactulose by transgalactosylation. Journal of Agricultural and Food Chemistry 56 557–563CrossRefGoogle ScholarPubMed
Matteuzzi, D, Swennen, E, Rossi, M, Hartman, T & Lebet, V 2004 Prebiotic effects of a wheat germ preparation in human healthy subjects. Food Microbiology 21 119–124CrossRefGoogle Scholar
Mendez, A & Olano, A 1979 Lactulose. A review of some chemical properties and application in infant nutrition and medicine. Dairy Sciences Abstracts 41 531–535Google Scholar
Onishi, N, Yamashiro, A & Yokozeki, K 1995 Production of galactooligosaccharide from lactose by Sterignatomyces elviae CBS8119. Applied and Environmental Microbiology 61 4022–4025Google Scholar
Panesar, PS, Panesar, R, Singh, RS, Kennedy, JF & Kumar, H 2006 Microbial production, immobilization and applications of β-galactosidase. Journal of Chemical Technology and Biotechnology 81 530–543CrossRefGoogle Scholar
Rycroft, CE, Jones, MR, Gibson, GR, Rastall, RA 2001 A comparative in vitro evaluation of the fermentation properties of prebiotic. Journal of Applied Microbiology 91 878–887Google Scholar
Salazar, N, Gueimonde, M, Hernández-Barranco, MH, Ruas-Madiedo, P & de los Reyes-Gavilan, CG 2008 Exopolysaccharides produced by intestinal Bifidobacterium strains act as fermentable substrates for human intestinal bacteria. Applied and Environmental Microbiology 74 4737–4745CrossRefGoogle ScholarPubMed
Sanz, ML, Gibson, GR & Rastall, RA 2005 Influence of disaccharide structure on prebiotic selectivity in vitro. Journal of Agricultural and Food Chemistry 53 5192–5199CrossRefGoogle ScholarPubMed
Shin, HJ & Yang, JW 1998 Enzymatic production of galactoligosacchatides by Bullera singularis β-galactosidase. Journal of Microbiology and Biotechnology 8 484–489Google Scholar
Splechtna, B, Nguyen, TH, Steinbock, M, Kulbe, KD, Lorenz, W & Haltrich, D 2006 Production of prebiotic galactooligosacchairdes from lactose using β-galactosidase from Lactobacillus reuteri. Journal of Agricultural and Food Chemistry 54 4999–5006CrossRefGoogle Scholar
Tomomatsu, H 1994 Health effects of oligosaccharides Food Technology 48 61–65Google Scholar
Topping, DL & Clifton, PM 2001 Short-chain fatty acids and human colonic function: roles of resistant starch and nonstarch polysaccharides. Physiological Reviews 81 1031–1064CrossRefGoogle ScholarPubMed
Tuohy, KM, Rouzaud, GCM, Brück, WM & Gibson, GR 2005 Modulation of the human gut microflora towards improved health using prebiotics-Assessment of efficacy. Current Pharmaceutical Design 11 75–90Google Scholar
Vanhoutte, T, De Preter, V, Brandt, E, Verbeke, K, Swings, J & Huys, G 2006 Molecular monitoring of the fecal microbiota of healthy human subjects during administration of lactulose and Saccharomyces boulardii. Environmental Microbiology 72 5990–5997CrossRefGoogle ScholarPubMed
Wong, JMW, Souza, de R, Kendall, CWC, Emam, A & Jenkins, DJA 2006 Colonic health: fermentation and short chain fatty acids. Journal of Clinical Gastroenterology 40 235–243CrossRefGoogle ScholarPubMed