Edinburgh Journal of Botany



ALLOZYME, SPORE AND FROND VARIATION IN SOME SCOTTISH POPULATIONS OF THE FERNS CYSTOPTERIS DICKIEANA AND CYSTOPTERIS FRAGILIS


J. C. PARKS a1, A. F. DYER a2 and S. LINDSAY a2
a1 Millersville University, PO Box 1002, Millersville, PA 17551-0302, USA
a2 Royal Botanic Garden Edinburgh, 20A Inverleith Row, Edinburgh EH3 5LR, UK

Abstract

Cystopteris dickieana R. Sim is a fern species that currently receives legal protection in Britain on the basis that it is endemic to Scotland and extremely rare. However, ever since it was first discovered in the 1830s, there has been considerable debate about its taxonomic status within the Cystopteris fragilis complex. This debate centres on the relative importance of two characters, the architecture of the fronds and the surface sculpturing of the spores, in delimiting C. dickieana from other taxa in the C. fragilis complex and is complicated by the fact that most comparative studies reputed to have included ‘C. dickieana’ have failed to include either the type material of C. dickieana or material from the ’type population‘ (i.e. the population of plants, of uniform and distinctive morphology, that still grows at the type locality for C. dickieana). The principal aim of this investigation was to generate some of the critical data and information currently missing from the debate concerning the taxonomic status of C. dickieana by including material from the ’type population‘ in an allozyme study of several Scottish Cystopteris populations. The frond architecture, spore sculpturing, and allozyme banding patterns were investigated in five natural and two cultivated populations of the C. fragilis complex. All plants from the ‘type population’ of C. dickieana had the distinctive frond architecture and rugose spores of the type material. Two populations comprised plants that had the frond architecture and echinate spores typical of C. fragilis. Also examined were two populations containing plants that had frond architecture typical of C. fragilis but rugose spores similar to those of the type material of C. dickieana. These plants occurred with typical C. fragilis in one of the populations and with typical C. dickieana in the other. In the latter population, situated close to the ‘type population’ for C. dickieana, a third, ‘smooth’ spore type occurred in nine out of 55 fertile plants. Consistent and analysable allozyme results were obtained for seven enzymes at ten loci: eight loci were polymorphic and all but the smallest population showed variation at one or more loci. In total, 22 allozyme phenotypes were recorded in 226 individuals. No consistent correlation was found between frond architecture, spore sculpturing and allozyme banding pattern. Our results provide no support for the recognition of C. dickieana as a distinct species related to C. fragilis.

(Received January 7 1999)
(Accepted October 18 1999)


Key Words: Cystopteris baenitzii; spore bank; spore sculpturing; spore type; conservation; frond architecture.