Journal of the Marine Biological Association of the UK



Short Communication

Inactivity of glycyl-glycyl-arginine and two putative (QSAR) peptide analogues of barnacle waterborne settlement pheromone


Anthony S.  Clare a1a2 and Mizue  Yamazaki a2
a1 Department of Marine Sciences and Coastal Management, University of Newcastle upon Tyne, Newcastle upon Tyne, Tyne & Wear, NE1 7RU, UK
a2 Marine Biological Association, Citadel Hill, Plymouth, PL1 2PB, UK

Abstract

The effect of two putative tripeptide analogues (isoleucine-isoleucine-arginine [IIR] and valine-leucine-arginine [VLR]) and a reported analogue, glycyl-glycyl-arginine (GGR), of the waterborne cue to settlement of Balanus amphitrite amphitrite Darwin cypris larvae has been investigated. Settlement in the presence of these tripeptides was not significantly different from that in filtered seawater. Because crude waterborne cue and settlement-inducing protein complex both significantly evoked settlement, the cyprids used in this study were competent to respond. The overall results, therefore, demonstrate that IIR, VLR and GGR are not analogues of barnacle waterborne settlement pheromone.

(Received October 8 1999)
(Accepted February 10 2000)