Plant Genetic Resources

Research Article

Molecular diversity in the Ukrainian melon collection as revealed by AFLPs and microsatellites

Padmavathi Nimmakayalaa1, Yan R. Tomasona1a2, Jooha Jeonga1, Gopinath Vajjaa1, Amnon Levia3, Paul Gibsona2a4 and Umesh K. Reddya1 c1

a1 Department of Biology and Gus R. Douglas Institute, West Virginia State University, Institute, WV 25112, USA

a2 Department of Selection and Seed Production, Dnepropetrovsk State Agrarian University, Voroshilov 25, Dnepropetrovsk 49600, Ukraine, e-mail: yantomason75@mail.ru

a3 US Vegetable Laboratory, USDA, ARS, 2875 Savannah Highway, Charleston, SC 29414, USA

a4 Department of Plant, Soil and Agriculture Systems, Southern Illinois University, 62901 Carbondale, IL, USA, e-mail: pgibson@siu.edu

Abstract

Thirty-eight melon accessions, which are of primary breeding importance in the Ukraine, were analysed for diversity. These collections represent a major non-US and non-western Europe source of melon germplasm that have not yet been subjected to molecular characterization. Molecular diversity was estimated based on a robust set of 465 polymorphisms gathered by amplified fragment length polymorphisms and simple sequence repeats (SSR). In this paper, we report 12 newly developed polymorphic SSR primer pairs, and their use for molecular characterization in the Ukrainian melon collections. Based on these polymorphisms, we estimated similarity indices that ranged from 0.70 to 1.00 among various accessions. The phylogenetic tree based on the similarity indices and a three-dimensional plot of the first three vectors of the principal component analysis corresponded fairly well with the existing three classical morphotypes namely aestivalis, europeus and hiemalis, under the convar Europeus, which is also known as adana. The polymorphisms generated in the current study, which are specific to the grouping of fruit types and days to maturity will be very useful for further genetic studies and marker-assisted selections.

(Received July 01 2008)

(Accepted September 21 2008)

(Online publication October 10 2008)

Correspondence:

c1 Corresponding author. E-mail: ureddy@wvstateu.edu

Footnotes

These two authors contributed equally to this paper.

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