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Molecular investigations of the stalked barnacle Vulcanolepas osheai and the epibiotic bacteria from the Brothers Caldera, Kermadec Arc, New Zealand

Published online by Cambridge University Press:  15 May 2009

Yohey Suzuki ,
Masae Suzuki ,
Shinji Tsuchida ,
Ken Takai ,
Koki Horikoshi ,
Alan J. Southward ,
William A. Newman  and
Toshiyuki Yamaguchi
Yohey Suzuki*
Affiliation:
Research Institute for Geo-resources & Environment, National Institute of Advanced Industrial Science & Technology (AIST), 1-1-1 Higashi, Tsukuba 305-8567, Japan
Masae Suzuki
Affiliation:
Extremobiosphere Research Center, Japan Agency for Marine-Earth Science & Technology, 2-15 Natsushima-cho, Yokosuka 237-0061, Japan
Shinji Tsuchida
Affiliation:
Extremobiosphere Research Center, Japan Agency for Marine-Earth Science & Technology, 2-15 Natsushima-cho, Yokosuka 237-0061, Japan
Ken Takai
Affiliation:
Extremobiosphere Research Center, Japan Agency for Marine-Earth Science & Technology, 2-15 Natsushima-cho, Yokosuka 237-0061, Japan
Koki Horikoshi
Affiliation:
Extremobiosphere Research Center, Japan Agency for Marine-Earth Science & Technology, 2-15 Natsushima-cho, Yokosuka 237-0061, Japan
Alan J. Southward
Affiliation:
Marine Biological Association of the United Kingdom, Citadel Hill, Plymouth, PLI 2PB Devon, UK
William A. Newman
Affiliation:
Scripps Institution of Oceanography, University of California at San Diego, La Jolla, CA 92093-0202, USA
Toshiyuki Yamaguchi
Affiliation:
Marine Biosystems Research Center, Chiba University, 1-33, Yayogi-cho, Inage, Chiba 263-8522, Japan
*
Correspondence should be addressed to: Y. Suzuki, Research Institute for Geo-resources & Environment, National Institute of Advanced Industrial Science & Technology (AIST), 1-1-1 Higashi, Tsukuba 305-8567, Japan email: yohey-suzuki@aist.go.jp
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Abstract

The hydrothermal-vent barnacle Vulcanolepas osheai of the subfamily Neolepadinae is one of the most conspicuous organisms at the Brothers Caldera, south Kermadec Arc, New Zealand. Like a neolepad species found in the Lau Basin, V. osheai harbours filamentous bacteria on its elongated cirral setae. To define the phylogenetic affiliation of the epibiotic bacteria and the nutrition of the barnacle host, we conducted molecular phylogenetic and isotopic analyses. Analysis of 16S rRNA gene sequences of microbial communities on the cirral setae showed that among 91 bacterial sequences investigated, 28 sequences were related to the ɛ-proteobacterial endosymbiont of Alviniconcha aff. hessleri; 11 sequences were related to the epibiont of the bresiliid shrimp Rimicaris exoculata. Fluorescence in situ hybridization showed that in contrary to results from the 16S rRNA gene-sequence library, approximately 80% of the filamentous bacteria hybridized with a probe targeting the sequences related to the epibiont of the bresiliid shrimp R. exoculata. The fatty-acid profiles of the filamentous bacteria and the host barnacle both contained high levels of monounsaturated C16 and C18 fatty acids, and the carbon isotopic compositions of the biomass and monounsaturated C16 and C18 fatty acids of both the bacteria and barnacle were nearly identical. This would suggest that the nutrition of the barnacle is highly dependent on bacteria thriving around the barnacle, including the epibiotic bacteria.

Keywords

molecular investigationsVulcanolepas osheaiepibiotic bacteriaBrothers Calderasouth Kermadec ArcNew Zealand
Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 89 , Issue 4 , June 2009 , pp. 727 - 733
Copyright
Copyright © Marine Biological Association of the United Kingdom 2009

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