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Genome fingerprinting of Salmonella typhi by pulsed-field gel electrophoresis for subtyping common phage types

Published online by Cambridge University Press:  19 October 2009

S. Nair ,
C. L. Poh ,
Y. S. Lim ,
L. Tay  and
K. T. Goh
S. Nair
Affiliation:
Department of Microbiology, Faculty of Medicine, National University of Singapore, Kent Ridge, Singapore 0511
C. L. Poh*
Affiliation:
Department of Microbiology, Faculty of Medicine, National University of Singapore, Kent Ridge, Singapore 0511
Y. S. Lim
Affiliation:
Enteric Bacteriology Laboratory, Department of Pathology, Singapore General Hospital and
L. Tay
Affiliation:
Enteric Bacteriology Laboratory, Department of Pathology, Singapore General Hospital and
K. T. Goh
Affiliation:
Quarantine and Epidemiology Department, Ministry of the Environment, Singapore
*
*Author for correspondence.
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The genomic DNA of 39 strains of Salmonella typhi isolated from local residents and patients who had visited countries in the Asian region was analysed for restriction fragment length polymorphisms (RFLP). Pulsed-field gel electrophoretic (PFGE) analysis of Xba I- and Spe I-generated genomic restriction fragments established 22 PFGE types whereas phage typing differentiated the 39 isolates into 9 distinct phage types. This study showed that PFGE is more discriminatory than phage typing as it is capable of subtyping S. typhi strains of the same phage types. Genetic relatedness among the isolates was determined. Seven major clusters were identified at SABSof > 0–80 and the remaining 13 isolates were distributed into minor clusters which were related at SABS of less than O.80. In conclusion, PFGE analysis in conjunction with distance matrix analysis served as a useful tool for delineating common S. typhi phage types of diverse origins from different geographical localesand separated in time.

Type
Research Article
Information
Epidemiology & Infection , Volume 113 , Issue 3 , December 1994 , pp. 391 - 402
Copyright
Copyright © Cambridge University Press 1994

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