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Comparison of techniques for demonstrating antibodies to Rift Valley fever virus

Published online by Cambridge University Press:  19 October 2009

R. Swanepoel
Affiliation:
Department of Virology, University of the Witwatersrand and National Institute for Virology, Sandringham 2131, Republic of South Africa
J. K. Struthers
Affiliation:
Department of Virology, University of the Witwatersrand and National Institute for Virology, Sandringham 2131, Republic of South Africa
M. J. Erasmus
Affiliation:
Department of Virology, University of the Witwatersrand and National Institute for Virology, Sandringham 2131, Republic of South Africa
S. P. Shepherd
Affiliation:
Department of Virology, University of the Witwatersrand and National Institute for Virology, Sandringham 2131, Republic of South Africa
G. M. McGillivray
Affiliation:
Department of Virology, University of the Witwatersrand and National Institute for Virology, Sandringham 2131, Republic of South Africa
B. J. Erasmus
Affiliation:
Veterinary Research Institute, Onderstepoort 0110, Republic of South Africa
B. J. H. Barnard
Affiliation:
Veterinary Research Institute, Onderstepoort 0110, Republic of South Africa
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Nine serological techniques were compared by monitoring the response to infection with Rift Valley fever (RVF) virus in three sheep. Antibodies were monitored daily for the first 14 days after infection, then weekly and later fortnightly up to week 24. The earliest antibody response was detected in one sheep on day 3 by a plaque reduction neutralization test, and by day 6 antibodies were demonstrable in all three sheep by haemagglutination-inhibition, reversed passive haemagglutination-inhibition, immunodiffusion, indirect immunofluorescence (IF), enzyme-linked immunosorbent assay and neutralization of cytopathic effect in cell cultures. Antibodies were demonstrable by complement fixation on day 8 at the earliest. IF and the two neutralization techniques produced the highest titres, but all tests could be used satisfactorily for the serological diagnosis of RVF. Inactivated antigen could be used for all except the neutralization tests. A radioimmunoassay technique using 125I-labelIed staphylococcal protein A detected antibodies on day 8 at the earliest and produced lower mean titres than some of the other techniques. This was probably because sheep immunoglobulins bind protein A poorly.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1986

References

Alexander, R. A. (1951). Rift Valley fever in the Union. Journal of the South African Veterinary Medical Association 22, 105109.Google Scholar
Ardoin, P., Clarke, D. H. & Hannoun, C. (1969). The preparation of arbovirus haemagglutinin by sonication and trypsin treatment. American Journal of Tropical Medicine and Hygiene 18, 592598.CrossRefGoogle ScholarPubMed
Bradstreet, C. M. P. & Taylor, C. E. D. (1962). Technique of complement-fixation tests applicable to the diagnosis of virus diseases. Monthly Bulletin of the Ministry of Health and the Public Health Laboratory Service 21, 96104.Google Scholar
Brès, P. (1981). Prevention of the spread of Rift Valley fever from the African continent. Contributions to Epidemiology and Biostatistics 3, 178190.Google Scholar
Casals, J. (1978). Rapid diagnosis of arboviral and similar infections of man: Rift Valley fever in Egypt, 1977. Journal of the Egyptian Public Health Association 53, 209215.Google ScholarPubMed
Christie, G. J. (1969). Rift Valley fever. Rhodesia Science News 3, 238240.Google Scholar
Clarke, D. H. (1964). Further studies on antigenic relationships among the viruses of the group B tick-borne complex. Bulletin of the World Health Organisation 31, 4556.Google Scholar
Clarke, D. H. & Casals, J. (1958). Techniques for haemagglutination and haemagglutination inhibition with arthropod-borne viruses. American Journal of Tropical Medicine and Hygiene 7, 561573.CrossRefGoogle ScholarPubMed
Cleveland, P. H., Richman, D. D., Oxman, M. N., Wickham, M. G., Binder, P. S. & Worthen, D. M. (1979). Immobilization of viral antigens on filter paper for a [125I]staphylococcal protein A immunoassay: a rapid and sensitive technique for detection of Herpes simplex virus antigens and antiviral antibodies. Journal of Immunological Methods 29, 360386.CrossRefGoogle ScholarPubMed
Cleveland, P. H., Richman, D. D., Oxman, M. N. & Worthen, D. M. (1982). Rapid serological technique for typing Herpes simplex viruses. Journal of Clinical Microbiology 15, 402407.CrossRefGoogle ScholarPubMed
Davies, F. G. (1975). Observations on the epidemiology of Rift Valley fever in Kenya. Journal of Hygiene 75, 219230.CrossRefGoogle ScholarPubMed
Eagle, H. (1959). Amino acid metabolism in mammalian cell cultures. Science 130, 432437.CrossRefGoogle ScholarPubMed
Earley, E., Peralta, P. H. & Johnson, K. M. (1967). A plaque neutralization method for arboviruses. Proceedings of the Society for Experimental Biology and Medicine 125, 741747.CrossRefGoogle ScholarPubMed
Hoogstraal, H. (1978). Rift Valley fever: an historical perspective. Journal of the Egyptian Public Health Association 53, 129135.Google ScholarPubMed
Imam, I. Z. E., Darwish, M. A. & El Karamany, R. (1979). An epidemic of Rift Valley fever in Egypt. 1. Diagnosis of Rift Valley fever in man. Bulletin of the World Health Organization 57, 437439.Google ScholarPubMed
Johnson, K. M. (1981). Summary of round table discussion [Workshop on Rift Valley fever, Herzlia, Israel, March 18–21,1980]. Contributions to Epidemiology and Biostatistics 3, 191193.Google Scholar
Johnson, K. M., Elliott, L. H. & Heyman, D. L. (1981). Preparation of polyvalent viral immunofluorescent intracellular antigens and use in human sero-surveys. Journal of Clinical Microbiology 14, 527529.CrossRefGoogle Scholar
Kärber, G. (1931). Beitrag zur kollektiven Behandlung pharmakologischer Reihensversuche. Archivfur experimented Pathologie und Pharmakologie 162, 480483.Google Scholar
Leibovitz, A. (1963). The growth and maintenance of tissue-cell cultures in free gas exchange with the atmosphere. American Journal of Hygiene 78, 173180.Google ScholarPubMed
Niklasson, B., Grandien, M., Peters, C. J. & Gargan, T. P. (1983). Detection of Rift Valley fever virus antigen by enzyme-linked immunosorbant assay. Journal of Clinical Microbiology 17, 10261031.CrossRefGoogle Scholar
Niklasson, B., Meegan, J. M. & Bengtsson, E. (1979). Antibodies to Rift Valley fever virus in Swedish U.N. soldiers in Egypt and the Sinai. Scandinavian Journal of Infectious Diseases 11, 313314.CrossRefGoogle ScholarPubMed
Niklasson, B., Peters, C. J., Grandien, M. & Wood, O. (1984). Detection of human immunoglobulin G and immunoglobulin M antibodies to Rift Valley fever virus by enzymelinked immunosorbant assay (ELISA). Journal of Clinical Microbiology 19, 225229.CrossRefGoogle Scholar
Richman, D. D., Cleveland, P. H., Oxman, M. N. & Johnson, K. M. (1982). The binding of staphylococcal protein A by the sera of different animal species. Journal of Immunology 128, 23002305.CrossRefGoogle ScholarPubMed
Richman, D. D., Cleveland, P. H., Oxman, M. N. & Zaia, J. A. (1981). A rapid radioimmunoassay using 125I-labelled staphylococcal protein A for antibody to varicella-zoster virus. Journal of Infectious Diseases 143, 693699.CrossRefGoogle Scholar
Shope, R. E. & Sather, G. E. (1979). Arboviruses. In Diagnostic Procedures for Viral, Rickettsial and Chlamydial Infections, 5th ed. (ed. Lennette, E. H. and Schmidt, N. J.), p. 791. Washington, D.C.American Public Health Association.Google Scholar
Struthers, J. K., Swanepoel, R. & Shepherd, S. (1984). Protein synthesis in Rift Valley fever virus-infected cells. Virology 134, 118124.CrossRefGoogle ScholarPubMed
Swanepoel, R. (1976). Studies on the epidemiology of Rift Valley fever. Journal of the South African Veterinary Association 47, 9394.Google ScholarPubMed
Swanepoel, R. (1981). Observations on Rift Valley fever in Zimbabwe. Contributions to Epidemiology and Biostatistics 3, 8391.Google Scholar
Swanepoel, R., Blackburn, N. K., Efstratiou, S. & Condy, J. B. (1978). Studies on Rift Valley fever in some African murids (Rodentia: Muridae). Journal of Hygiene 80, 183196.CrossRefGoogle ScholarPubMed
Swanepoel, R., Blackburn, N. K., Lander, K. P., Vickers, D. B. & Lewis, A. R. (1975). An investigation of infectious infertility and abortion of cattle. Rhodesian Veterinary Journal 6, 4255.Google Scholar
Swanepoel, R., Struthers, J. K. & McGillivray, G. M. (1983). Reversed passive haemagglutination and inhibition with Rift Valley fever and Crimean-Congo haemorrhagic fever and viruses. American Journal of Tropical Medicine and Hygiene 32, 610617.CrossRefGoogle Scholar
Zaia, J. A. & Oxman, M. N. (1977). Antibody to Varicella-Zoster virus-induced membrane antigen: immunofluorescence assay using monodisperse glutaraldehyde-fixed target cells. Journal of Infectious Diseases 136, 519530.CrossRefGoogle ScholarPubMed