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Recognition of the cryptic plasmid, pSLT, by restriction fingerprinting and a study of its incidence in Scottish salmonella isolates

Published online by Cambridge University Press:  19 October 2009

D. J. Brown ,
D. S. Munro  and
D. J. Platt
D. J. Brown
Affiliation:
University Department of Bacteriology, Glasgow Royal Infirmary, Castle Street, Glasgow G4 0SF
D. S. Munro
Affiliation:
Scottish Salmonella Reference Laboratory, Stobhill General Hospital, Glasgow G21 3UW
D. J. Platt
Affiliation:
University Department of Bacteriology, Glasgow Royal Infirmary, Castle Street, Glasgow G4 0SF
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The plasmid pSLT is a cryptic plasmid of 60 megadaltons (Md) present in Salmonella typhimurium LT2. We present evidence that it has a characteristic fingerprint when digested with the restriction enzymes PstI and SmaI. Among a representative collection of S. typhimurium isolates it was present in 67% of strains and was widely distributed amongst different phage types (DT) with the exception of DT10 and U285. Furthermore, its prevalence among veterinary isolates was significantly higher than among human isolates. It was not found among any of the 96 strains representative of other salmonella serotypes currently prevalent and thus appears to be serotype-specific.

Type
Research Article
Information
Journal of Hygiene , Volume 97 , Issue 2 , October 1986 , pp. 193 - 197
Copyright
Copyright © Cambridge University Press 1986

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