Mapping candidate genes for Drosophila melanogaster resistance to the parasitoid wasp Leptopilina boulardi
Drosophila melanogaster resistance against the parasitoid wasp Leptopilina boulardi is under the control of a single gene (Rlb), with two alleles, the resistant one being dominant. Using strains bearing deletions, we previously demonstrated that the 55E2–E6; 55F3 region on chromosome 2R is involved in the resistance phenomenon. In this paper, we first restricted the Rlb containing region by mapping at the molecular level the breakpoints of the Df(2R)Pc66, Df(2R)P34 and Df(2R)Pc4 deficiencies, using both chromosomal in situ hybridization and Southern analyses. The resistance gene was localized in a 100 kb fragment, predicted to contain about 10 different genes. Male recombination genetic experiments were then performed, leading to identification of two possible candidates for the Rlb gene. Potential involvement of one of this genes, edl/mae, is discussed.(Received August 14 2006)
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p1 Present address: Institut de Génétique et Microbiologie, Université Paris Sud, Bat. 400, 91405 Orsay cedex, France.
p2 Present address: UMR 1112 UNSA INRA ‘Réaction des Organismes aux Stress Environnementaux’, 400 route des Chappes – BP 167, 06903 Sophia-Antipolis, France.