Genetical Research

Mapping candidate genes for Drosophila melanogaster resistance to the parasitoid wasp Leptopilina boulardi

M. HITA a1, E. ESPAGNE a1p1, F. LEMEUNIER a2, L. PASCUAL a3, Y. CARTON a2, G. PERIQUET a1 and M. POIRIE a1p2c1
a1 Institut de Recherche sur la Biologie de l’Insecte – UMR CNRS 6035, Université François Rabelais, Parc Grandmont, 37200 Tours, France
a2 Laboratoire Populations, Génétique, Evolution CNRS, 91198 Gif sur Yvette, France
a3 Departamento de Genetica, Universidad de Valencia, 46100 Burjassot, Valencia, Spain

Article author query
hita m   [PubMed][Google Scholar] 
espagne e   [PubMed][Google Scholar] 
lemeunier f   [PubMed][Google Scholar] 
pascual l   [PubMed][Google Scholar] 
carton y   [PubMed][Google Scholar] 
periquet g   [PubMed][Google Scholar] 
poirie m   [PubMed][Google Scholar] 


Drosophila melanogaster resistance against the parasitoid wasp Leptopilina boulardi is under the control of a single gene (Rlb), with two alleles, the resistant one being dominant. Using strains bearing deletions, we previously demonstrated that the 55E2–E6; 55F3 region on chromosome 2R is involved in the resistance phenomenon. In this paper, we first restricted the Rlb containing region by mapping at the molecular level the breakpoints of the Df(2R)Pc66, Df(2R)P34 and Df(2R)Pc4 deficiencies, using both chromosomal in situ hybridization and Southern analyses. The resistance gene was localized in a 100 kb fragment, predicted to contain about 10 different genes. Male recombination genetic experiments were then performed, leading to identification of two possible candidates for the Rlb gene. Potential involvement of one of this genes, edl/mae, is discussed.

(Received August 14 2006)

c1 UMR 1112 UNSA INRA 400 route des Chappes, BP 167 – 06903 Sophia-Antipolis, France. Tel: (33) 4 92 38 6409. Fax: (33) 4 92 38 6655. e-mail:
p1 Present address: Institut de Génétique et Microbiologie, Université Paris Sud, Bat. 400, 91405 Orsay cedex, France.
p2 Present address: UMR 1112 UNSA INRA ‘Réaction des Organismes aux Stress Environnementaux’, 400 route des Chappes – BP 167, 06903 Sophia-Antipolis, France.