Hostname: page-component-7c8c6479df-24hb2 Total loading time: 0 Render date: 2024-03-28T07:35:11.018Z Has data issue: false hasContentIssue false

Use of ELISAs in field studies of rabbit haemorrhagic disease (RHD) in Australia

Published online by Cambridge University Press:  01 June 2000

B. D. COOKE
Affiliation:
CSIRO, Wildlife and Ecology, GPO Box 284, Canberra, ACT, Australia 2601
A. J. ROBINSON
Affiliation:
CSIRO, Wildlife and Ecology, GPO Box 284, Canberra, ACT, Australia 2601
J. C. MERCHANT
Affiliation:
CSIRO, Wildlife and Ecology, GPO Box 284, Canberra, ACT, Australia 2601
A. NARDIN
Affiliation:
Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia, Via Bianchi 7, 25124 Brescia, Italy
L. CAPUCCI
Affiliation:
Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia, Via Bianchi 7, 25124 Brescia, Italy
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

ELISA techniques developed for the veterinary diagnosis of Rabbit Haemorrhagic Disease (RHD) in domestic rabbits were used for studying the epidemiology of RHD in Australian wild rabbits. The combination of ELISA techniques that distinguished IgA, IgG and IgM antibody responses and a longitudinal data set, mainly based on capture-mark-recapture of rabbits, provided a reliable basis for interpreting serology and set the criteria used to classify rabbits' immunological status. Importantly, young with maternal antibodies, immune rabbits and rabbits apparently re-exposed to RHD were readily separated. Three outbreaks of RHD occurred in 1996–7. The timing of RHD outbreaks was mainly driven by recruitment of young rabbits that generally contracted RHD after they lost their maternally derived immunity. Young that lost maternal antibodies in summer were not immediately infected, apparently because transmission of RHDV slows at that time, but contracted RHD in the autumn when conditions were again suitable for disease spread.

Type
Research Article
Copyright
© 2000 Cambridge University Press