Epidemiology and Infection



Expanding multiple antibiotic resistance among clinical strains of Vibrio cholerae isolated from 1992–7 in Calcutta, India


P. GARG a1, S. CHAKRABORTY a1, I. BASU a1, S. DATTA a1, K. RAJENDRAN a1, T. BHATTACHARYA a1, S. YAMASAKI a1a2, S. K. BHATTACHARYA a1, Y. TAKEDA a3, G. BALAKRISH NAIR a1 and T. RAMAMURTHY a1c1
a1 National Institute of Cholera and Enteric Diseases, Calcutta-700010, India
a2 Research Institute, International Medical Center of Japan, Shijuku-ku, Tokyo 162–8655, Japan
a3 National Institute of Infectious Diseases, Shijuku-ku, Tokyo 162–8640, Japan

Abstract

Antimicrobial susceptibilities of Vibrio cholerae strains isolated from cholera patients admitted to the Infectious Diseases Hospital, Calcutta, India for 6 years were analysed to determine the changing trends; 840 V. cholerae strains isolated in 1992–1997 were included in this study. Among V. cholerae serogoup O1 and O139, ampicillin resistance increased from 1992 (35 and 70%, respectively) to 1997 (both serogroups 100%). Resistance to furazolidone and streptomycin was constantly high among V. cholerae O1 strains with gradual increase in resistance to other drugs such as ciprofloxacin, co-trimoxazole, neomycin and nalidixic acid. V. cholerae O139 strains exhibited susceptibilities to furazolidone and streptomycin comparable with those of O1 strains. However, after initial increase in resistance to chloramphenicol and co-trimoxazole, all the V. cholerae O139 strains became susceptible to these two drugs from 1995 onwards. Both V. cholerae O1 and O139 remained largely susceptible to gentamicin and tetracycline. V. cholerae non-O1, non-O139 strains, in contrast, exhibited high levels of resistance to virtually every class of antimicrobial agents tested in this study especially from 1995. Kruskal–Wallis one-way analysis showed that V. cholerae O1 Ogawa serogroup exhibited significant yearly increase in resistance to nine antibiotics followed by non-O1 non-O139 and O139 strains to six antibiotics and two antibiotics respectively. Interesting observation encountered in this study was the dissipation of some of the resistant patterns commonly found among V. cholerae non-O1 non-O139 or O1 serogroups to the O139 serogroup and vice versa during the succeeding years.

(Accepted December 20 1999)


Correspondence:
c1 Author for correspondence: National Institute of Cholera and Enteric Diseases, P-33, C.I.T. Road, Scheme XM, Beliaghata, Calcutta-700010, India.


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