Hostname: page-component-8448b6f56d-mp689 Total loading time: 0 Render date: 2024-04-19T18:58:44.970Z Has data issue: false hasContentIssue false
  • English
  • Français

Increased secretion of insulin-like growth factor I into milk of cows treated with recombinantly derived bovine growth hormone

Published online by Cambridge University Press:  01 June 2009

Colin G. Prosser ,
Ivan R. Fleet  and
Anthony N. Corps
Colin G. Prosser
Affiliation:
AFRC Institute of Animal Physiology and Genetics Research, Babraham, Cambridge CB2 4AT, UK
Ivan R. Fleet
Affiliation:
AFRC Institute of Animal Physiology and Genetics Research, Babraham, Cambridge CB2 4AT, UK
Anthony N. Corps
Affiliation:
AFRC Institute of Animal Physiology and Genetics Research, Babraham, Cambridge CB2 4AT, UK
Get access Rights & Permissions [Opens in a new window]

Summary

Six lactating, non-pregnant Jersey cows were given subcutaneous injections of recombinantly derived bovine growth hormone for 7 d. Milk yield was increased by 4·5 kg/d on d 7, compared with the average yield of 10·7 ± 0·4 kg/d (mean ± s. e. m.) for the 7d preceding treatment. Concentrations of insulin-like growth factor I (IGF-I) in the milk increased from 0·44 ± 0·04 nmol/1 (mean ± s. e. m.) during the 7 d preceding treatment to 1·6 ± 0·2 nmol/1 on d 7 of treatment. Taking the increase in milk yield into account the total increase in the secretion of IGF-I into milk of one udder half was 6-fold. Plasma concentrations of total IGF-I rose from 15·5 + 1·3 nmol/1 (mean ± s. e. m.) on the day preceding treatment to 56·9 ± 3·6 nmol/1 (mean ± s. e. m.) on d 7 of treatment. Mammary plasma flow increased from 1·6 ± 0·09 to 2·2 ± 0·06 1/min udder half over the same time. Estimates of the amount of IGF-I that reached the mammary gland gave values of 24 and 116 nmol/min udder half before and during treatment respectively. IGF-I in milk of treated cows was associated predominantly with proteins ranging from 40000 to 150000 mol. wt, but a significant proportion (19%) of the total IGF-I was present in the free unbound form. IGF-I crosslinking studies revealed the presence in milk of one specifically labelled band at 31000 mol. wt.

Type
Original Articles
Information
Journal of Dairy Research , Volume 56 , Issue 1 , February 1989 , pp. 17 - 26
Copyright
Copyright © Proprietors of Journal of Dairy Research 1989

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Bauman, D. E., Eppard, P. J., DeGeeter, M. J. & Lanza, G. M. 1985 Responses of high-producing dairy cows to long-term treatment with pituitary somatotropin and recombinant somatotropin. Journal of Dairy Science 68 1352–1362CrossRefGoogle ScholarPubMed
Baxter, R. C., Maitland, J. E., Raison, R. L., Reddel, R. R. & Sutherland, R. L. 1983 High molecular weight somatomedin-C/IGF-1 from T47D human mammary carcinoma cells: immunoreactivity and bioactivity. In Insulin-like Growth Factors/Somatomedins: basic chemistry, biology, clinical importance pp. 615–618 (Ed Spencer, E. M.). New York: De GruyterGoogle Scholar
Baxter, R. C. & Martin, J. L. 1986 Radioimmunoassay of growth hormone-dependent insulin-like growth factor binding protein in human plasma. Journal of Clinical Investigation 78 1504–1512CrossRefGoogle ScholarPubMed
Baxter, R. C., Zaltsman, Z. & Turtle, J. R. 1984 Immunoreactive somatomedin-C/insulin-like growth factor 1 and its binding protein in human milk. Journal of Clinical Endocrinology and Metabolism 58 955–959Google Scholar
Bickerstaffe, R., Annison, E. F. & Linzell, J. L. 1974 The metabolism of glucose, acetate, lipids and amino acids in lactating dairy cows. Journal of Agricultural Science 82 71–85CrossRefGoogle Scholar
Corps, A. N., Brown, K. D., Rees, L. H., Carr, J. & Prosser, C. G. 1988 The insulin-like growth factor 1 content in human milk increases between early and full lactation. Journal of Clinical Endocrinology and Metabolism 67 25–29.CrossRefGoogle ScholarPubMed
Davis, S. R., Gluckman, P. D., Hart, I. C. & Henderson, H. V. 1987 Effects of injecting growth hormone or thyroxine on milk production and blood plasma concentrations of insulin-like growth factors 1 and II in dairy cows. Journal of Endocrinology 114 17–24CrossRefGoogle ScholarPubMed
Eppard, P. J., Bauman, D. E. & McCutcheon, S. N. 1985 Effect of dose of bovine growth hormone on lactation of dairy cows. Journal of Dairy Science 68 1109–1115Google Scholar
Ethier, S. P., Kudla, A. & Cundiff, K. C. 1987 Influence of hormone and growth factor interactions on the proliferative potential of normal rat mammary epithelial cells in vitro. Journal of Cellular Physiology 132 161–167CrossRefGoogle ScholarPubMed
Francis, O. L., Read, L. C., Ballard, F. J., Bagley, C. J., Upton, F. M., Gravestock, P. M. & Wallace, J. C. 1986 Purification and partial sequence analysis of insulin-like growth factor-1 from bovine colostrum. Biochemical Journal 233 207–213CrossRefGoogle ScholarPubMed
Gertler, A., Cohen, N. & Maoz, A. 1983 Human growth hormone but not ovine or bovine growth hormones exhibits galactopoietic prolactin-like activity in organ culture from bovine lactating mammary gland. Molecular and Cellular Endocrinology 33 169–182.Google Scholar
Goodman, G. T., Akers, R. M., Friderici, K. H. & Tucker, H. A. 1983 Hormonal regulation of α- lactalbumin secretion from bovine mammary tissue cultured in vitro. Endocrinology 112 1324–1330CrossRefGoogle ScholarPubMed
Grant, M. B., Schmetz, I., Russell, B., Hahwood, H. J., Silverstein, J. & Merimee, T. J. 1986 Changes in insulin-like growth factors I and II and their binding protein after a single intramuscular injection of growth hormone. Journal of Clinical Endocrinology and Metabolism 63 981–984CrossRefGoogle ScholarPubMed
Hardouin, S., Hossenlopp, P., Segovia, B., Seurin, D., Portolan, G., Lassarre, C. & Binoux, M. 1987 Heterogeneity of insulin-like growth factor binding proteins and relationships between structure and affinity. I. Circulating forma in man. European Journal of Biochemistry 170 121–132CrossRefGoogle Scholar
Hart, I. C., Flux, D. S., Andrews, P. & McNeilly, A. S. 1975 Radioimmunoassay for ovine and caprine growth hormone: its application to the measurement of basal circulating levels of growth hormone in the goat. Hormone and Metabolic Research 7 35–40Google Scholar
Honegger, A. & Humbel, R. E. 1986 Insulin-like growth factors I and II in fetal and adult bovine serum. Purification, primary structures, and immunological cross-reactivities. Journal of Biological Chemistry 261 569–575CrossRefGoogle Scholar
Huff, K. K., Kaufman, D., Gabbay, K. H., Spencer, E. M., Lippman, M. E. & Dickson, R. B. 1986 Secretion of an insulin-like growth factor-1-related protein by human breast cancer cells. Cancer Research 46 4613–4619Google Scholar
Imagawa, W., Spencer, E. M., Larson, L. & Nandi, S. 1986 Somatomedin-C substitutes for insulin for the growth of mammary epithelial cells from normal virgin mice in serum-free collagen gel cell culture. Endocrinology 119 2695–2699Google Scholar
Johnsson, I. D. & Hart, I. C. 1986 Manipulation of milk yield with growth hormone. In Recent Advances in Animal Nutrition, pp. 105–123 (Eds Haresign, W. and Cole, D. J. A.). London: ButterworthsGoogle Scholar
Laemmli, U. K. 1970 Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227 680–685CrossRefGoogle ScholarPubMed
Linzell, J. L. 1966 Measurement of venous flow by continuous thermodilution and its application to measurement of mammary blood flow in the goat. Circulation Research 18 745–754CrossRefGoogle ScholarPubMed
Malven, P. V., Head, H. H., Collier, R. J. & Buonomo, F. C. 1987 Periparturient changes in secretion and mammary uptake of insulin and in concentrations of insulin and insulin-like growth factors in milk of dairy cows. Journal of Dairy Science 70 2254–2265CrossRefGoogle ScholarPubMed
McDowell, G. H., Hart, I. C. & Kirby, A. C. 1987 Local intra-arterial infusion of growth hormone into the mammary glands of sheep and goats: effects on milk yield and composition, plasma hormones and metabolites. Australian Journal of Biological Sciences 40 181–189CrossRefGoogle ScholarPubMed
Murphy, L. J., Bell, G. I. & Friesen, H. G. 1987 Tissue distribution of insulin-like growth factor I and II messenger ribonucleic acid in the adult rat. Endocrinology 120 1279–1282CrossRefGoogle ScholarPubMed
Peel, C. J. & Bauman, D. E. 1987 Somatotropin and lactation. Journal of Dairy Science 70 474–486Google Scholar
Prosser, C. G., Sankakan, L., Hennighausen, L. & Topper, Y. J. 1987 a Comparison of the roles of insulin and insulin-like growth factor 1 in casein gene expression and in the development of α-lactalbumin and glucose transport activities in the mouse mammary epithelial cell. Endocrinology 120 1411–1416CrossRefGoogle ScholarPubMed
Prosser, C. G., Davis, A. J., Fleet, I. R., Rees, L. H. & Heap, R. B. 1987 b Mechanism of transfer of IGF-I into milk. Journal of Endocrinology 115 (suppl.) 91Google Scholar
Prosser, C. G., Fleet, I. R., Hart, I. C. & Heap, R. B. 1987 c Changes in concentration of insulin-like growth factor 1 (IGF-1) in milk during bovine growth hormone treatment in the goat. Journal of Endocrinology 112 (suppl.) 65Google Scholar
Prosser, C. G., Fleet, I. R., Corps, A. N., Heap, R. B. & Froesch, E. R. 1988 Increase in milk secretion and mammary blood flow in a close intra-arterial infusion of insulin-like growth factor 1 into the mammary gland of the goat. Journal of Endocrinology 117 (Suppl.) 248Google Scholar
Škarda, J., Urbanová, E., Becka, S., Houdebine, L. M., Delouis, C., Pichova, D., Picha, J. & Bilek, J. 1982 Effect of bovine growth hormone on development of goat mammary tissue in organ culture. Endocrinologia Experimentalis 16 19–31Google Scholar
Wilkins, J. R. & D' Ercole, A. J. 1985 Affinity-labeled plasma somatomedin-C/insulin like growth factor 1 binding proteins. Evidence of growth hormone dependence and subunit structure. Journal of Clinical Investigation 75 1350–1358Google Scholar
Winder, S. J. & Forsyth, I. A. 1986 Insulin-like growth factor 1 (IGF-1) is a potent mitogen for ovine mammary epithelial cells. Journal of Endocrinology 108 (suppl.) 141Google Scholar