Peptidyl transferase activity catalyzed by protein-free 23S ribosomal RNA remains elusive
In two recent articles by Nitta et al. (1998a, 1998b), evidence was presented that in vitro-transcribed protein-free 23S rRNA of Escherichia coli (and its individual domains) can catalyze peptide bond formation. The implications of these studies are considerable because demonstration of catalytic activity associated with protein-free 23S rRNA would be fundamental to our understanding of the mechanism and evolution of protein synthesis (Noller et al., 1992; Khaitovich et al., 1999).(Accepted February 12 1999)
Key Words: peptide bond formation; ribosome; RNA catalysis; rRNA.
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