The identification and characterization of a novel splicing protein, Isy1p, of Saccharomyces cerevisiae
We have identified a novel splicing factor, Isy1p, through two-hybrid screens for interacting proteins involved in nuclear pre-mRNA splicing. Isy1p was tagged and demonstrated to be part of the splicing machinery, associated with spliceosomes throughout the splicing reactions. At least a portion of the Isy1 protein population is associated with snRNAs; low levels of U5 and U6 snRNAs are coimmunoprecipitated specifically with Isy1p. When the ISY1 gene was knocked out, no defect in vegetative growth was observed. Using a sensitive in vivo splicing assay, however, we observed lower splicing efficiency in the isy1 null mutant compared to wild-type, indicating that Isy1p is important in the optimization of splicing.(Received August 17 1998)
(Revised September 10 1998)
(Accepted November 23 1998)
Key Words: pre-mRNA splicing; snRNA; snRNPs; spliceosome; yeast.
c1 Reprint requests to: Jean D. Beggs, Institute of Cell and Molecular Biology, King's Buildings, Mayfield Road, Edinburgh EH9 3JR, United Kingdom; e-mail: email@example.com.