a1 ARC-Onderstepoort Veterinary Institute, Private Bag X5, Onderstepoort 0110, South Africa
a2 University of Pretoria, Faculty of Veterinary Science, Pretoria 0110, South Africa
a3 Jozini State Veterinary Office, Private Bag X004, Jozini 3969, South Africa
The polymerase chain reaction was used to detect trypanosomes in samples collected from cattle, wild animals and tsetse flies in KwaZulu-Natal Province, South Africa. A total of 673 samples from cattle and 266 from tsetse flies in the study area located near the Hluhluwe-Umfolozi Game Reserve were analysed. Both Trypanosoma congolense and T. vivax were found as single or mixed infections in cattle and tsetse flies. Moreover, the T. congolense in the infections were found to comprise 2 genotypic groups: the Savannah-type and the Kilifi-type, which were present either as single or mixed infections in cattle and in tsetse flies.
(Received November 21 2008)
(Revised December 18 2008)
(Accepted December 19 2008)
(Online publication March 02 2009)