The Journal of Agricultural Science

Table of Contents - 1994 - Volume 122, Issue 02  

Animals

A cranial mesenteric vein preparation for measurement of amino acid uptake by lambs

S. A. Neutzea1, V. H. Oddya2 and J. M. Goodena1

a1 Department of Animal Science, University of Sydney, Camden, NSW 2570, Australia

a2 Elizabeth MacArthur Agricultural Institute, NSW Agriculture, PMB 8, Camden, NSW 2570, Australia

Abstract

A surgical preparation was developed to measure uptake of amino acids from the small intestine into the cranial mesenteric vein (CMV) of lambs. Results from this preparation were compared with those from the traditional hepatic portal vein (PV) preparation in the same lambs. Necropsy revealed that, in contrast to the PV preparation which included all portal-drained viscera, the CMV preparation drained primarily small intestine (0·84–0·92 small intestinal mass). The CMV preparation contributed 0·238 of blood flow from and 0·307 of oxygen consumption by the PV preparation. Venous-arterial differences for alpha-amino nitrogen (AAN), phenylalanine (Phe) and tyrosine (Tyr) were three- to fourfold greater in the CMV than in the PV preparation, indicating that the CMV preparation is more sensitive for measuring amino acid uptake. The ratio CMV/PV for uptake of AAN was 0·61 (P< 0·001), but this ratio was much closer to unity (mean 0·84) for Phe and Tyr. Since the CMV preparation drains primarily the small intestine, through which all amino acid absorption occurs, it should prove to be extremely valuable for studying absorption from and metabolism within this organ using a combination of arteriovenous and isotopic tracer techniques.

(Received August 10 1993)

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