Epidemiology and Infection

Research Article

Seroprevalence of leptospirosis in a rural flood prone district of Bangladesh

M. G. Morsheda1, H. Konishia1 c1, Y. Teradaa1, Y. Arimitsua2 and T. Nakazawaa1

a1 Department of Microbiology, Yamaguchi University School of Medicine, 1144 Kogushi, Ube, Yamaguchi 755, Japan

a2 Department of bacteriology, National Institute of Health, 1-23-1 Toyama, Shinjuku, Tokyo 162, Japan

Abstract

Leptospirosis is a worldwide zoonotic disease. In the present investigation, a total of 89 human sera from a flood prone district of Bangladesh was screened by a one-point microscapsule agglutination test (MCAT). MCAT-positive and-doubtful sera were further tested by microscopic agglutination test (MAT) against 16 reference serovars of Leptospira interrogans. and the antibody titres determined. In MCAT, 34 sera were positive and 22 were doubtful. Among those positive and doubtful sera. 33 and 20. respectively were tested by MAT. Thirty-four out of 53 MCAT-screened samples were MAT-positive. The titres ranged from 20 to 1600 with antibodies to serovars copenhageni, australis, cynopteri and icterohaemorrhagiae being the most prevalent. Eleven MCAT-positive samples failed to react with any strains used by MAT, suggesting the presence of new or untested serovars. Among the MAT-positive samples, the presence of antibody against two or more serovars was more common than that of a single serovar. The present study suggests that rural people in Bangladesh are at high risk to leptospiral infection.

(Accepted January 24 1994)

Correspondence:

c1 Corresponding author and reprints request to: H. Konishi, Department of Microbiology. Yamaguchi University School of Medicine. 1144 Kogushi. Ube. Yamaguchi 755. Japan.

Metrics