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Sialic acid content and surface hydrophobicity of group B streptococci

Published online by Cambridge University Press:  15 May 2009

L. A. Teixeira ,
A. M. S. Figueiredo ,
B. T. Ferreira ,
V. M. L. Alves ,
P. E. Nagao ,
C. S. Alviano ,
J. Angluster ,
F. C. Silva-Filho  and
L. C. Benchetrit
L. A. Teixeira
Affiliation:
Instituto de Microbiologia
A. M. S. Figueiredo*
Affiliation:
Instituto de Microbiologia
B. T. Ferreira
Affiliation:
Instituto de Microbiologia
V. M. L. Alves
Affiliation:
Instituto de Microbiologia
P. E. Nagao
Affiliation:
Instituto de Microbiologia
C. S. Alviano
Affiliation:
Instituto de Microbiologia
J. Angluster
Affiliation:
Instituto de Microbiologia
F. C. Silva-Filho
Affiliation:
Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Cidade Universitária, 21941, Rio de Janeiro, Brazil
L. C. Benchetrit
Affiliation:
Instituto de Microbiologia
*
*Corresponding author
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The sialic acid content and the cell-surface hydrophobicity index of 40 group B streptococci (GBS) strains were assessed. GBS isolated from invasive infections (virulent strains) presented an increased level of sialic acid content (1.4%) when compared with GBS isolated from asymptomatic patients (0.53%). Treatment of GBS strain 85634 with neuraminidase resulted in a decrease (about 25%) in the net negative surface charge as assessed by cell electrophoresis. This finding suggests that sialic acid residues are important anionogenic groups exposed on GBS cell surface. N-acetylneuraminic acid was the only sialic acid derivative characterized in the strain 85634 as evaluated by gas-liquid chromatography. GBS from different serotypes presented a hydrophobic index mean value of 0.9. Even though the sialic acid contributed effectively to the negative charge on GBS cell surface, no difference was observed in the hydrophobic index when virulent and avirulent strains were compared.

Type
Research Article
Information
Epidemiology & Infection , Volume 110 , Issue 1 , February 1993 , pp. 87 - 94
Copyright
Copyright © Cambridge University Press 1993

References

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