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Correlation of change in phage type with pulsed field profile and 16S rrn profile in Salmonella enteritidis phage types 4, 7 and 9a

Published online by Cambridge University Press:  15 May 2009

N. G. Powell
Affiliation:
Laboratory of Enteric Pathogens, Central Public Health Laboratory, 61 Colindate Avenue, London NW9 5HT, UK
E. J. Threlfall*
Affiliation:
Laboratory of Enteric Pathogens, Central Public Health Laboratory, 61 Colindate Avenue, London NW9 5HT, UK
H. Chart
Affiliation:
Laboratory of Enteric Pathogens, Central Public Health Laboratory, 61 Colindate Avenue, London NW9 5HT, UK
S. L. Schofield
Affiliation:
Laboratory of Enteric Pathogens, Central Public Health Laboratory, 61 Colindate Avenue, London NW9 5HT, UK
B. Rowe
Affiliation:
Laboratory of Enteric Pathogens, Central Public Health Laboratory, 61 Colindate Avenue, London NW9 5HT, UK
*
* Corresponding author.
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Using pulsed-field gel electrophoresis (PFGE) and 16S rRNA (rrn) analysis (ribotyping). the in viro derivation of strains of Salmonella enteritidis PTs 9a and 7 from a strain of S. enteritidis PT 4 has been demonstrated. All strains were isolated from a single patient over a 6-week period. Further studies have demonstrated that in terms of pulsed-field profile and ribotype, the genotypes of the patient-derived strains differed from those of the reference strains of the respective phage types. It is concluded that when used in combination, these methods can provide evidence of phylogenetic relationships in apparently unrelated S. enteritidis phage types isolated during pathogenesis of disease.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1995

References

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